Latrunculin B or ATP Depletion Induces Cofilin-dependent Translocation of Actin into Nuclei of Mast Cells
| Autor: | Brian Pope, Alan G. Weeds, Annmarie Pendleton, Anna Koffer |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Phalloidine
Protein Conformation Active Transport Cell Nucleus Arp2/3 complex Apoptosis macromolecular substances Microfilament Biochemistry Adenosine Triphosphate medicine Animals Mast Cells Molecular Biology Cells Cultured Actin Fluorescent Dyes Cell Nucleus Microscopy Confocal biology Rhodamines Microfilament Proteins Actin remodeling Cell Biology Cofilin Bridged Bicyclo Compounds Heterocyclic Flow Cytometry Actins Rats Cell biology Thiazoles Cell nucleus medicine.anatomical_structure Actin Depolymerizing Factors Profilin biology.protein Thiazolidines Calcium Marine Toxins MDia1 |
| Zdroj: | Journal of Biological Chemistry. 278:14394-14400 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m206393200 |
| Popis: | Increasing cellular G-actin, using latrunculin B, in either intact or permeabilized rat peritoneal mast cells, caused translocation of both actin and an actin regulatory protein, cofilin, into the nuclei. The effect was not associated with an increase in the proportion of apoptotic cells. The major part of the nuclear actin was not stained by rhodamine-phalloidin but could be visualized with an actin antibody, indicating its monomeric or a conformationally distinct state, e.g. cofilin-decorated filaments. Introduction of anti-cofilin into permeabilized cells inhibited nuclear actin accumulation, implying that an active, cofilin-dependent, import exists in this system. Nuclear actin was localized outside the ethidium bromide-stained region, in the extrachromosomal nuclear domain. In permeabilized cells, the appearance of nuclear actin and cofilin was not significantly affected by increasing [Ca(2+)] and/or adding guanosine 5'-O-(3-thiotriphosphate), but was greatly promoted when ATP was withdrawn. Similarly, ATP depletion in intact cells also induced nuclear actin accumulation. In contrast to the effects of latrunculin B, ATP depletion was associated with an increase in cortical F-actin. Our results suggest that the presence of actin in the nucleus may be required for certain stress-induced responses and that cofilin is essential for the nuclear import of actin. |
| Databáze: | OpenAIRE |
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