Ox-LDL Upregulates IL-6 Expression by Enhancing NF-κB in an IGF2-Dependent Manner in THP-1 Macrophages
| Autor: | Qian Wang, Xin Ma, Jing-Jing Zhao, Yan-Chao Wang, Jia-Yi Zhao, Jing-Bo Lu, Chuan Huang, Yu-Rong Qiu, Yan-Hua Sha, Lei Zheng, Shu-Fen Li, Ji-Juan Gao, Yan-Wei Hu |
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| Rok vydání: | 2015 |
| Předmět: |
Small interfering RNA
medicine.medical_treatment Immunology Macrophage-activating factor Transfection Cell Line chemistry.chemical_compound Downregulation and upregulation Insulin-Like Growth Factor II medicine Humans Immunology and Allergy RNA Messenger Interleukin 6 Messenger RNA Dose-Response Relationship Drug biology Interleukin-6 Macrophages Growth factor NF-kappa B NF-κB Molecular biology Up-Regulation Lipoproteins LDL Cytokine chemistry biology.protein RNA Interference lipids (amino acids peptides and proteins) Signal Transduction |
| Zdroj: | Inflammation. 38:2116-2123 |
| ISSN: | 1573-2576 0360-3997 |
| DOI: | 10.1007/s10753-015-0194-1 |
| Popis: | Interleukin 6 (IL-6) is a pro-inflammatory cytokine that is well established as a vital factor in determining the risk of coronary heart disease and pathogenesis of atherosclerosis. Moreover, accumulating evidences have shown that oxidized low-density lipoprotein (ox-LDL) can promote IL-6 expression in macrophages. Nevertheless, the underlying mechanism of how ox-LDL upregulates IL-6 expression remains largely unexplained. We found that the expression of insulin-like growth factor 2 (IGF2), nuclear factor kappa B (NF-κB), and IL-6 was upregulated at both the messenger RNA (mRNA) and protein levels in a dose-dependent manner when treated with 0, 25, 50, or 100 μg/mL of ox-LDL for 48 h in THP-1 macrophages. Moreover, overexpression of IGF2 significantly upregulated NF-κB and IL-6 expressions in THP-1 macrophages. However, the upregulation of NF-κB and IL-6 expressions induced by ox-LDL were significantly abolished by IGF2 small interfering RNA (siRNA) in THP-1 macrophages. Further studies indicated the upregulation of IL-6 induced by ox-LDL could be abolished when treated with NF-κB siRNA in THP-1 macrophages. Ox-LDL might upregulate IL-6 in the cell and its secretion via enhancing NF-κB in an IGF2-dependent manner in THP-1 macrophages. |
| Databáze: | OpenAIRE |
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