The Role of Dafachronic Acid Signaling in Development and Longevity in Caenorhabditis elegans: Digging Deeper Using Cutting-Edge Analytical Chemistry
| Autor: | Michael Witting, Hugo Aguilaniu, Paola Fabrizio |
|---|---|
| Přispěvatelé: | École normale supérieure de Lyon (ENS de Lyon), Helmholtz Zentrum München = German Research Center for Environmental Health, École normale supérieure - Lyon (ENS Lyon), Helmholtz-Zentrum München (HZM) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Caenorhabditis Elegans
Aging Analytical Chemistry Steroid Hormones Steroid Signaling Pathway 0301 basic medicine steroid hormones steroid signaling pathway Endocrinology Diabetes and Metabolism medicine.medical_treatment Review Steroid biosynthesis Bioinformatics lcsh:Diseases of the endocrine glands. Clinical endocrinology 01 natural sciences Steroid 03 medical and health sciences Endocrinology medicine Caenorhabditis elegans Organism ComputingMilieux_MISCELLANEOUS lcsh:RC648-665 biology [SDV.BA]Life Sciences [q-bio]/Animal biology 010401 analytical chemistry biology.organism_classification Phenotype 0104 chemical sciences Cell biology Steroid hormone 030104 developmental biology Signal transduction Hormone |
| Zdroj: | Frontiers in Endocrinology Frontiers in Endocrinology, 2016, 7, ⟨10.3389/fendo.2016.00012⟩ Front. Endocrin. 7:12 (2016) Frontiers in Endocrinology, Vol 7 (2016) Frontiers in Endocrinology, Frontiers, 2016, 7, ⟨10.3389/fendo.2016.00012⟩ |
| ISSN: | 1664-2392 |
| DOI: | 10.3389/fendo.2016.00012⟩ |
| Popis: | Steroid hormones regulate physiological processes in species ranging from plants to humans. A wide range of steroid hormones exist, and their contributions to processes such as growth, reproduction, development, and aging, is almost always complex. Understanding the biosynthetic pathways that generate steroid hormones and the signaling pathways that mediate their effects is thus of fundamental importance. In this work, we review recent advances in (i) the biological role of steroid hormones in the roundworm Caenorhabditis elegans and (ii) the development of novel methods to facilitate the detection and identification of these molecules. Our current understanding of steroid signaling in this simple organism serves to illustrate the challenges we face moving forward. First, it seems clear that we have not yet identified all of the enzymes responsible for steroid biosynthesis and/or degradation. Second, perturbation of steroid signaling affects a wide range of phenotypes, and subtly different steroid molecules can have distinct effects. Finally, steroid hormone levels are critically important, and minute variations in quantity can profoundly impact a phenotype. Thus, it is imperative that we develop innovative analytical tools and combine them with cutting-edge approaches such as comprehensive and highly selective liquid chromatography coupled to mass spectrometry (LC-MS) based or new methods such as supercritical fluid chromatography coupled to mass spectrometry (SFC-MS) if we are to obtain a better understanding of the biological functions of steroid signaling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|