Immunoblotting on HEp-2 cells increases the detection of antitopoisomerase 1 antibodies in patients with systemic sclerosis
| Autor: | Bernard Weill, Amélie Servettaz, Mathieu C. Tamby, Nicolas Tamas, Joseph Reinbolt, Alice Bérezné, Luc Mouthon, Philippe Guilpain, Loïc Guillevin, Frédéric Batteux |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Adult
Systemic disease Anti-nuclear antibody Centromere Immunoblotting Immunology Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Autoantigens Sensitivity and Specificity Scleroderma Cell Line Immunopathology medicine Humans Immunology and Allergy Autoantibodies Autoimmune disease Scleroderma Systemic biology business.industry Topoisomerase Middle Aged medicine.disease Connective tissue disease DNA Topoisomerases Type I Antibodies Antinuclear Immunoglobulin G biology.protein Antibody business |
| Zdroj: | Clinical Immunology. 123:82-88 |
| ISSN: | 1521-6616 |
| DOI: | 10.1016/j.clim.2006.12.003 |
| Popis: | In order to improve the detection of antitopoisomerase 1 antibodies in a cohort of 111 systemic sclerosis patients, we have performed immunoblots on protein extracts of HEp-2 cells. Using indirect immunofluorescence and ELISA, 27 patients (24.3%) had antitopoisomerase 1 antibodies, 32 (28.8%) had anticentromere antibodies, 31 (27.9%) had antinuclear antibodies with none of these two antibodies and 21 (18.9%) had no antinuclear antibody. IgG from 24/27 (88.9%) patients with antitopoisomerase 1 antibodies bound to 2 protein bands of 63 and 100 kDa identified as topoisomerase 1 by N-terminal sequencing. Antitopoisomerase 1 antibodies were detected in 9 (8.1%) patients who had no antitopoisomerase 1 antibody as determined by ELISA. Patients with antitopoisomerase 1 antibodies had an almost similar phenotype without distinction between ELISA or immunoblot approaches. Our results provide evidence for the use of a combination of ELISA and immunoblot approaches for the detection of antitopoisomerase 1 antibodies. |
| Databáze: | OpenAIRE |
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