Improvement on citric acid production in solid-state fermentation by Aspergillus niger LPB BC mutant using citric pulp
| Autor: | Carlos Ricardo Soccol, Cristine Rodrigues, Juliana Teodoro, Ashok Pandey, Luciana Porto de Souza Vandenberghe |
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| Rok vydání: | 2008 |
| Předmět: |
Citrus
Nitrogen Ultraviolet Rays Carbohydrates Bioengineering engineering.material Applied Microbiology and Biotechnology Biochemistry Citric Acid chemistry.chemical_compound Animals Food science Molecular Biology Strain (chemistry) biology Pulp (paper) Aspergillus niger Substrate (chemistry) Water General Medicine Hydrogen-Ion Concentration biology.organism_classification Kinetics chemistry Solid-state fermentation Alcohols Fermentation Mutation engineering Salts Methanol Citric acid Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 158(1) |
| ISSN: | 1559-0291 |
| Popis: | Citric acid (CA) production has been conducted through a careful strain selection, physical-chemical optimization and mutation. The aim of this work was to optimize the physical-chemical conditions of CA production by solid-state fermentation (SSF) using the Aspergillus niger LPB BC strain, which was isolated in our laboratory. The parental and mutant strain showed a good production of CA using citric pulp (CP) as a substrate. The physical-chemical parameters were optimized and the best production was reached at 65% moisture, 30 degrees C and pH 5.5. The influence of the addition of commercial and alternative sugars, nitrogen sources, salts, and alcohols was also studied. The best results (445.4 g of CA/kg of CP) were obtained with sugarcane molasses and 4% methanol (v/w). The mutagenesis induction of LPB BC was performed with UV irradiation. Eleven mutant strains were tested in SSF where two mutants showed a higher CA production when compared to the parental strain. A. niger LPB B3 produced 537.6 g of CA/kg of CP on the sixth day of fermentation, while A. niger LPB B6 produced 616.5 g of CA/kg of CP on the fourth day of fermentation, representing a 19.5% and 37% gain, respectively. |
| Databáze: | OpenAIRE |
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