Elevated levels of Interleukin (IL)-33 induce bone pathology but absence of IL-33 does not negatively impact normal bone homeostasis
| Autor: | Heather A Bullock, Hana E. Baker, Matthew J Hamang, Angela J. Okragly, Y. Linda Ma, Jonathan Lucchesi, Andrea P. Martin, Robert J. Benschop, Emily A. Pena |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Bone density Bone pathology Immunology Osteoclasts Biochemistry Collagen Type I Bone resorption Proinflammatory cytokine Mice 03 medical and health sciences 0302 clinical medicine N-terminal telopeptide Bone Density Osteoclast Internal medicine medicine Animals Immunology and Allergy Femur Bone Resorption Molecular Biology Mice Knockout Lumbar Vertebrae business.industry Interleukin Hematology Interleukin-33 Mice Inbred C57BL 030104 developmental biology Endocrinology medicine.anatomical_structure Cytokines Female Cortical bone Peptides business 030215 immunology |
| Zdroj: | Cytokine. 79:66-73 |
| ISSN: | 1043-4666 |
| Popis: | Interleukin (IL)-33 is a member of the IL-1 family. IL-33 effects are mediated through its receptor, ST2 and IL-1RAcP, and its signaling induces the production of a number of pro-inflammatory mediators, including TNFα, IL-1β, IL-6, and IFN-γ. There are conflicting reports on the role of IL-33 in bone homeostasis, with some demonstrating a bone protective role for IL-33 whilst others show that IL-33 induces inflammatory arthritis with concurrent bone destruction. To better clarify the role IL-33 plays in bone biology in vivo, we studied IL-33 KO mice as well as mice in which the cytokine form of IL-33 was overexpressed. Mid-femur cortical bone mineral density (BMD) and bone strength were similar in the IL-33 KO mice compared to WT animals during the first 8months of life. However, in the absence of IL-33, we observed higher BMD in lumbar vertebrae and distal femur in female mice. In contrast, overexpression of IL-33 resulted in a marked and rapid reduction of bone volume, mineral density and strength. Moreover, this was associated with a robust increase in inflammatory cytokines (including IL-6 and IFN-γ), suggesting the bone pathology could be a direct effect of IL-33 or an indirect effect due to the induction of other mediators. Furthermore, the detrimental bone effects were accompanied by increases in osteoclast number and the bone resorption marker of C-terminal telopeptide collagen-I (CTX-I). Together, these results demonstrate that absence of IL-33 has no negative consequences in normal bone homeostasis while high levels of circulating IL-33 contributes to pathological bone loss. |
| Databáze: | OpenAIRE |
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