Ocelot and oncilla spermatozoa can bind hen egg perivitelline membranes
| Autor: | Leanes Cruz da Silva, Rafael de Morais Garay, Thyara de Deco-Souza, Antonio Carlos Csermak-Júnior, C.F. Letícia Bergo, Saullo Vinicius Pereira Alves, Gediendson Ribeiro de Araujo, Tarcízio Antônio Rego de Paula |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
endocrine system Felidae Sperm binding Vitelline membrane Semen Biology Cryopreservation Andrology Leopardus pardalis Endocrinology Food Animals parasitic diseases Animals Sperm quality reproductive and urinary physiology Sperm-Ovum Interactions Leopardus tigrinus urogenital system General Medicine Sperm Spermatozoa Membrane Immunology Animal Science and Zoology Chickens Vitelline Membrane Semen Preservation |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 1873-2232 |
| Popis: | We evaluated the capacity of ocelot and oncilla spermatozoa to bind to the perivitelline membranes (PVMs) of hen eggs in a sperm binding assay (S-PVM). In addition, a device that improves the standardization of the assay was developed. The number of sperm bound to the PVM in fresh (T1) and frozen–thawed (T2) semen from both species was compared to the sperm quality observed in routine tests. The PVM was stretched on a circular silicone device to create a standardized area for analysis. In both treatments and for both species, the spermatozoa were able to bind to the PVM, indicating that PVM may be used for a sperm binding assay in ocelot and oncilla. The S-PVM assay did not differ in fresh and frozen–thawed ocelot sperm (p > 0.05). However, fewer oncilla sperm (p < 0.05) were bound to the PVM in T2, indicating that the proposed test may be able to detect injuries that compromise sperm binding abilities. The device maintained the PVM stretched during the processing and defined the evaluation area. |
| Databáze: | OpenAIRE |
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