Accumulation of extracellular RGR-d in Bruch's membrane and close association with drusen at intercapillary regions
| Autor: | Harold Kochounian, Lincoln V. Johnson, Henry K.W. Fong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Aging genetic structures Adolescent Retinal Drusen Retinal Pigment Epithelium Drusen Biology Bruch's membrane Article Receptors G-Protein-Coupled Cellular and Molecular Neuroscience chemistry.chemical_compound Young Adult Extracellular medicine Humans Protein Isoforms Retinal G protein coupled receptor Eye Proteins Aged Aged 80 and over Retinal pigment epithelium Retinal Anatomy medicine.disease Sensory Systems eye diseases Ophthalmology medicine.anatomical_structure Tissue sections chemistry Splice isoforms sense organs Bruch Membrane Extracellular Space |
| Popis: | Human retinal pigment epithelial (RPE) cells synthesize an extraneous splice isoform of retinal G protein-coupled receptor (RGR). In this study, we analyzed the exon-skipping variant of RGR (RGR-d) that is found in extracellular deposits. RPE-choroid tissue sections were prepared from postmortem human eyes from donors of various ages. RGR-d was analyzed in drusen and Bruch's membrane by immunohistochemical localization. Extracellular RGR-d is present in most drusen, including hard, soft, confluent and early-stage. Initial drusen formation is known to be preferentially associated with the intercapillary regions of Bruch's membrane. We corroborated this significant association of drusen, including early-stage drusen, with the intercapillary regions. The distribution of extracellular RGR-d in Bruch's membrane differs in old and young donors. In older persons, nodes of concentrated RGR-d accumulate at intercapillary loci, predominantly at the lateral edges of the capillaries of the choriocapillaris. RGR-d loci at the lateral capillary wall appear numerous in old, but not young, donors. Intensely immunostained RGR-d loci can be found at the base of early-stage drusen mounds in the older donors and may precede the formation of these drusen. |
| Databáze: | OpenAIRE |
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