Activation of T helper cells in sentinel node predicts poor prognosis in oral squamous cell carcinoma
| Autor: | Eva Munck-Wikland, Valtteri Häyry, Lars-Olaf Cardell, Eric Hjalmarsson, Gregori Margolin, Susanna Kumlien Georén, Pedro Farrajota Neves da Silva, Ola Winqvist, Krzysztof Piersiala, Åsa Kågedal |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Oncology medicine.medical_treatment Lymphadenopathy 0302 clinical medicine Head and neck cancer education.field_of_study Multidisciplinary Oral cancer Standard treatment T-Lymphocytes Helper-Inducer Middle Aged Sentinel node Prognosis Lymphatic system Lymphoid tissues Lymphatic Metastasis 030220 oncology & carcinogenesis Tumour immunology Medicine Female Sentinel Lymph Node Adult medicine.medical_specialty Science Immunology Adaptive immunity Population Article Disease-Free Survival 03 medical and health sciences Internal medicine medicine Humans education Survival analysis Aged Neoplasm Staging Sweden Sentinel Lymph Node Biopsy Squamous Cell Carcinoma of Head and Neck business.industry Neck dissection medicine.disease Clinical trial 030104 developmental biology Leukocytes Mononuclear Neoplasm Recurrence Local business CD8 |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-79273-3 |
| Popis: | Recurrence in oral squamous cell carcinoma (OSCC) significantly reduces overall survival. Improved understanding of the host’s immune status in head and neck cancer may facilitate identification of patients at higher risk of recurrence and improve patients’ selection for ongoing clinical trials assessing the effectiveness of immune checkpoint inhibitors (CPI). We aimed to investigate Sentinel Node-derived T-cells and their impact on survival. We enrolled prospectively 28 OSCC patients treated at Karolinska University Hospital, Stockholm, Sweden with primary tumour excision and elective neck dissection. On top of the standard treatment, the enrolled patients underwent sentinel node procedure. T cells derived from Sentinel nodes, non-sentinel nodes, primary tumour and PBMC were analyzed in flow cytometry. Patients who developed recurrence proved to have significantly lower level of CD4+ CD69+ in their sentinel node (31.38 ± 6.019% vs. 43.44 ± 15.33%, p = 0.0103) and significantly higher level of CD8+ CD HLA-DR+ (38.95 ± 9.479% vs. 24.58 ± 11.36%, p = 0.0116) compared to disease-free individuals. Survival analysis of studied population revealed that patients with low proportion of CD4+ CD69+ had significantly decreased disease-free survival (DFS) of 19.7 months (95% CI 12.6–26.9) compared with 42.6 months (95% CI 40.1–45.1) in those with high CD4+ CD69+ proportion in their Sentinel Nodes (log-rank test, p = 0.033). Our findings demonstrate that characterization of T-cell activation in Sentinel Node serves as a complementary prognostic marker. Flow cytometry of Sentinel Node may be useful in both patients’ surveillance and selection for ongoing CPI clinical trials in head and neck cancer. |
| Databáze: | OpenAIRE |
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