Metabolism of 25-hydroxyvitamin D3 by rat kidney cells in culture: isolation and identification of cis- and trans-19-nor-10-oxo-25-hydroxyvitamin D3
| Autor: | Robert U. Simpson, Heinrich K. Schnoes, Hector F. DeLuca, Herbert E. Paaren, Joseph K. Wichmann |
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| Rok vydání: | 1984 |
| Předmět: |
Male
Biophysics Stereoisomerism Biology Kidney Biochemistry chemistry.chemical_compound Biotransformation In vivo polycyclic compounds medicine Animals Molecular Biology Cells Cultured Chromatography High Pressure Liquid Calcifediol Binding protein Spectrum Analysis Metabolism Rats medicine.anatomical_structure chemistry Chickens Cis–trans isomerism |
| Zdroj: | Archives of biochemistry and biophysics. 230(1) |
| ISSN: | 0003-9861 |
| Popis: | A primary confluent culture of epithelial cells from rat kidney has been developed. These cells possess a 3.2-3.4 S high-affinity, low-capacity binding protein for 1,25-dihydroxyvitamin D3. They metabolize 25-hydroxyvitamin D3 to at least five metabolites. Two have been identified as 1,25-dihydroxyvitamin D3 and 24,25-dihydroxyvitamin D3. Two others have been identified by means of physical data and cochromatography as trans 19-nor-10-oxo-25-hydroxyvitamin D3 and the other as its cis isomer. These two "metabolites" have not been observed in vivo, but one of them (cis) comigrates with 1,25-dihydroxyvitamin D3 on straight-phase high-performance liquid chromatography. Thus, mere cochromatography on high-performance liquid chromatography is not sufficient to identify critical vitamin D metabolites. |
| Databáze: | OpenAIRE |
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