Prominin-1/CD133: Lipid Raft Association, Detergent Resistance, and Immunodetection
| Autor: | Jana Karbanová, Martin Bornhäuser, Christine A. Fargeas, Aurelio Lorico, Denis Corbeil |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Surface Markers Association (object-oriented programming) Detergents Cell Computational biology Biology 03 medical and health sciences Membrane Microdomains Cancer stem cell Cancer Stem Cells Prominin-1 medicine Humans AC133 Antigen Cancer/Leukemia General expression Lipid raft Translational medicine Cell Biology General Medicine Neural/Progenitor Stem Cells Adult Stem Cells 030104 developmental biology medicine.anatomical_structure Hematopoietic Stem/Progenitor Cells Neoplastic Stem Cells Renal Stem Cells Stem cell Hematopoietic Regeneration Biomarkers Perspectives Developmental Biology |
| Zdroj: | Stem Cells Translational Medicine |
| ISSN: | 2157-6580 2157-6564 |
| DOI: | 10.1002/sctm.17-0223 |
| Popis: | Summary The cell surface antigen prominin-1 (alias CD133) has gained enormous interest in the past 2 decades and given rise to debates as to its utility as a biological stem and cancer stem cell marker. Important and yet often overlooked knowledge that is pertinent to its physiological function has been generated in other systems given its more general expression beyond primitive cells. This article briefly discusses the importance of particular biochemical features of CD133 with relation to its association with membrane microdomains (lipid rafts) and proper immunodetection. It also draws attention toward the adequate use of detergents and caveats that may apply to the interpretation of the results generated. |
| Databáze: | OpenAIRE |
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