Improved silica-guanidiniumthiocyanate DNA isolation procedure based on selective binding of bovine alpha-casein to silica particles
| Autor: | Jaap Goudsmit, Pauline M. E. Wertheim-van Dillen, J. F. L. Weel, R. Boom, Marcel Beld, C. J. A. Sol |
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| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
Silicon dioxide Restriction Mapping Guanidines Polymerase Chain Reaction Sensitivity and Specificity chemistry.chemical_compound Casein Lysis buffer Animals Humans DNA Primers chemistry.chemical_classification Caseins Bacteriology Buffer solution Silicon Dioxide DNA extraction Enzyme Biochemistry chemistry Virus Diseases Radioimmunoprecipitation assay buffer DNA Viral Cattle Thiocyanates DNA Protein Binding |
| Zdroj: | Journal of clinical microbiology, 37(3), 615-619. American Society for Microbiology |
| ISSN: | 0095-1137 |
| Popis: | DNA purified from clinical cerebrospinal fluid and urine specimens by a silica-guanidiniumthiocyanate procedure frequently contained an inhibitor(s) of DNA-processing enzymes which may have been introduced by the purification procedure itself. Inhibition could be relieved by the use of a novel lysis buffer containing alpha-casein. When the novel lysis buffer was used, alpha-casein was bound by the silica particles in the first step of the procedure and eluted together with DNA in the last step, after which it exerted its beneficial effects for DNA-processing enzymes. In the present study we have compared the novel lysis buffer with the previously described lysis buffer with respect to double-stranded DNA yield (which was nearly 100%) and the performance of DNA-processing enzymes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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