Eugenol confers resistance to Tomato yellow leaf curl virus (TYLCV) by regulating the expression of SlPer1 in tomato plants
| Autor: | Zhiqi Shi, Xiaofang Hang, Wen-Jing Lv, Jian Chen, Yanfeng Xue, Li-Na Li, Gan Yin, Wei-Jie Sun |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nitroprusside DNA Complementary Transcription Genetic Bioengineering Endogeny Nitric Oxide 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Solanum lycopersicum Plant Growth Regulators Transcription (biology) Sequence Analysis Protein Complementary DNA Botany Eugenol Tomato yellow leaf curl virus Amino Acid Sequence Hydrogen Sulfide Cloning Molecular Promoter Regions Genetic Molecular Biology Phylogeny Disease Resistance Plant Diseases Plant Proteins biology Base Sequence Inoculation fungi food and beverages General Medicine R gene biology.organism_classification Plant Leaves 030104 developmental biology chemistry Begomovirus Salicylic Acid Salicylic acid 010606 plant biology & botany Biotechnology |
| Zdroj: | New biotechnology. 33(3) |
| ISSN: | 1876-4347 |
| Popis: | Tomato yellow leaf curl virus (TYLCV) is one of the most devastating plant diseases, and poses a significant agricultural concern because of the lack of an efficient control method. Eugenol is a plant-derived natural compound that has been widely used as a food additive and in medicine. In the present study, we demonstrated the potential of eugenol to enhance the resistance of tomato plants to TYLCV. The anti-TYLCV efficiency of eugenol was significantly higher than that of moroxydine hydrochloride (MH), a widely used commercial antiviral agent. Eugenol application stimulated the production of endogenous nitric oxide (NO) and salicylic acid (SA) in tomato plants. The full-length cDNA of SlPer1, which has been suggested to be a host R gene specific to TYLCV, was isolated from tomato plants. A sequence analysis suggested that SlPer1 might be a nucleobase-ascorbate transporter (NAT) belonging to the permease family. The transcript levels of SlPer1 increased markedly in response to treatment with eugenol or TYLCV inoculation. The results of this study also showed that SlPer1 expression was strongly induced by SA, MeJA (jasmonic acid methyl ester), and NO. Thus, we propose that the increased transcription of SlPer1 contributed to the high anti-TYLCV efficiency of eugenol, which might involve in the generation of endogenous SA and NO. Such findings provide the basis for the development of eugenol as an environmental-friendly agricultural antiviral agent. |
| Databáze: | OpenAIRE |
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