DJ-1 isoforms in whole blood as potential biomarkers of Parkinson disease
| Autor: | Thomas J. Montine, Xiangmin Lin, Shu-Ching Hu, Min Shi, Jing Zhang, Kevin C. Cain, David R. Goodlett, Harvey Checkoway, Elaine R. Peskind, James B. Leverenz, Cyrus P. Zabetian, John Scott Edgar, Travis J. Cook, Catherine Pan, Brad A. Racette |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Gene isoform medicine.medical_specialty Blotting Western Protein Deglycase DJ-1 Disease Biology medicine.disease_cause Article 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid Alzheimer Disease Internal medicine medicine Humans Protein Isoforms Electrophoresis Gel Two-Dimensional Aged 030304 developmental biology Whole blood Oncogene Proteins Synucleinopathies 0303 health sciences Multidisciplinary Intracellular Signaling Peptides and Proteins Parkinson Disease Middle Aged medicine.disease 3. Good health Endocrinology Immunology Female Alzheimer's disease Protein Processing Post-Translational Biomarkers 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep00954 |
| Popis: | DJ-1 is a multifunctional protein that plays an important role in oxidative stress, cell death, and synucleinopathies, including Parkinson disease. Previous studies have demonstrated that total DJ-1 levels decrease in the cerebrospinal fluid, but do not change significantly in human plasma from patients with Parkinson disease when compared with controls. In this study, we measured total DJ-1 and its isoforms in whole blood of patients with Parkinson disease at various stages, Alzheimer disease, and healthy controls to identify potential peripheral biomarkers of PD. In an initial discovery study of 119 subjects, 7 DJ-1 isoforms were reliably detected, and blood levels of those with 4-hydroxy-2-nonenal modifications were discovered to be altered in late-stage Parkinson disease. This result was further confirmed in a validation study of another 114 participants, suggesting that, unlike total DJ-1 levels, post-translationally modified isoforms of DJ-1 from whole blood are candidate biomarkers of late-stage Parkinson disease. |
| Databáze: | OpenAIRE |
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