The human Y chromosome homologue of XG: transcription of a naturally truncated gene
| Autor: | Nathan A. Ellis, Ricky Critcher, Peter N. Goodfellow, James German, Polly A. Weller |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Primates DNA Complementary X Chromosome Transcription Genetic Pseudogene Molecular Sequence Data Pseudoautosomal region Biology Y chromosome Polymerase Chain Reaction Homology (biology) Gene mapping Y Chromosome Genetics Animals Humans Promoter Regions Genetic Molecular Biology In Situ Hybridization Fluorescence Genetics (clinical) X chromosome Genomic organization Base Sequence Chromosome Mapping Exons General Medicine Molecular biology Alternative Splicing Testis determining factor Blood Group Antigens Cell Adhesion Molecules Pseudogenes |
| Zdroj: | Human Molecular Genetics. 4:859-868 |
| ISSN: | 1460-2083 0964-6906 |
| Popis: | The XG blood group gene spans PABX1, the pseudo-autosomal boundary on the X chromosome. The firstthree exons are pseudoautosomal and the remainingseven are X-speciflc. On the Y chromosome SRY andRPS4Y are located In Y-speclfic sequences within70 kb of the boundary. Transcription from the XGpromoter on the Y chromosome has been detectedby cDNA cloning and PCR-based methods. Splicingof the pseudoautosomal exon 3 of XG occurs tomultiple sites in Y-speciflc sequences. Transcriptsdetected include antlsense SRY sequences andXG-RPS4Yhybrid transcripts. The heterogeneity andlow abundance of transcripts as well as the lack ofmaintenance of the XG open reading frame in all butone transcript argue against a specific Y-chromosomegene product. An expressed pseudogene of XG,XGPY, has been mapped to interval Yq11.21. XGPYis transcribed and subject to alternative splicing.Sequence comparison suggests that XGPVoriginatedfrom XG by a gene duplication event In the primatelineage.INTRODUCTIONThe pseudoautosomal region is composed of sequences thatare shared by the X and Y chromosomes, and crossing overoccurs between these sequences during male meiosis (1,2).One pseudoautosomal region, PAR], is situated at the distalends of the short arms of the X and Y chromosomes (see ref.3, for review), and a second pseudoautosomal region is situatedat the distal ends of the X and Y chromosome long arms (4).The pseudoautosomal boundary {PABXY1) is defined as thejunction of pseudoautosomal and sex chromosome-specificsequences, and it marks the proximal limit of PAR] beyondwhich male recombination does not normally occur. At thepseudoautosomal boundary there is an abrupt transition fromthe homologous sequences in PAR] to non-homologoussequences in the X- and Y-specific regions (5).The polymorphic blood group gene XG, which maps to thedistal part of the X-specific region of Xp (6,7), encodesthe cell surface antigen Xg |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|