Assembly and operation of an open-source, computer numerical controlled (CNC) robot for performing cranial microsurgical procedures

Autor: Mathew L. Rynes, Samantha Linn, Daniel Sousa Schulman, Peter Sherman, Suhasa B. Kodandaramaiah, Leila Ghanbari, Michael Laroque, Judith Dominguez, Zahra S. Navabi
Rok vydání: 2020
Předmět:
Zdroj: Nat Protoc
ISSN: 1750-2799
1754-2189
DOI: 10.1038/s41596-020-0318-4
Popis: Cranial microsurgery is an essential procedure for accessing the brain through the skull that can be used to introduce neural probes that measure and manipulate neural activity. Neuroscientists have typically used tools such as high-speed drills adapted from dentistry to perform these procedures. As the number of technologies available for neuroscientists has increased, the corresponding cranial microsurgery procedures to deploy them have become more complex. Using a robotic tool that automatically performs these procedures could standardize cranial microsurgeries across neuroscience laboratories and democratize the more challenging procedures. We have recently engineered a robotic surgery platform that utilizes principles of computer numerical control (CNC) machining to perform a wide variety of automated cranial procedures. Here, we describe how to adapt, configure and use an inexpensive desktop CNC mill equipped with a custom-built surface profiler for performing CNC-guided microsurgery on mice. Detailed instructions are provided to utilize this 'Craniobot' for performing circular craniotomies for coverslip implantation, large craniotomies for implanting transparent polymer skulls for cortex-wide imaging access and skull thinning for intact skull imaging. The Craniobot can be set up in
Databáze: OpenAIRE
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