Persistence of Ebola virus in semen among Ebola virus disease survivors in Sierra Leone: A cohort study of frequency, duration, and risk factors

Autor: Barbara Knust, Wenbo Xu, Nathalie Broutet, A. Coursier, Thomas Massaquoi, Ute Ströher, Kyle T. Bernstein, Philippe Gaillard, Kara N. Durski, Yong Zhang, Jaclyn E. Marrinan, Pierre Formenty, N. Habib, Wei Jia Liu, Shalini Singaravelu, Suzanna L. R. McDonald, Guizhen Wu, H. Liu, Elizabeth Ervin, F. Yamba, Archchun Ariyarajah, Foday Sahr, Anna Thorson, Gibrilla F. Deen, M. Carino, Margaret Lamunu, F. R. Sesay
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Male
RNA viruses
Viral Diseases
Physiology
Epidemiology
Molecular biology
Pathology and Laboratory Medicine
Molecular biology assays and analysis techniques
medicine.disease_cause
Geographical locations
Persistence (computer science)
Cohort Studies
Medical Conditions
0302 clinical medicine
Risk Factors
Medicine and Health Sciences
Survivors
030212 general & internal medicine
education.field_of_study
Nucleic acid analysis
General Medicine
Middle Aged
RNA analysis
Ebolavirus
Body Fluids
Viral Persistence and Latency
Blood
Infectious Diseases
Medical Microbiology
Filoviruses
Viral Pathogens
Viruses
RNA
Viral
Medicine
Pathogens
Anatomy
Ebola Virus
Research Article
Neglected Tropical Diseases
Cohort study
Adult
Sexual transmission
030231 tropical medicine
Population
Semen
Real-Time Polymerase Chain Reaction
Microbiology
Ebola Hemorrhagic Fever
Sierra Leone
Sierra leone
03 medical and health sciences
Virology
medicine
Humans
education
Microbial Pathogens
Aged
Viral Hemorrhagic Fevers
Ebola virus
Biology and life sciences
Hemorrhagic Fever Viruses
business.industry
Organisms
Hemorrhagic Fever
Ebola
Tropical Diseases
Research and analysis methods
Molecular biology techniques
Medical Risk Factors
Africa
People and places
business
Demography
Zdroj: PLoS Medicine, Vol 18, Iss 2, p e1003273 (2021)
PLoS Medicine
ISSN: 1549-1676
1549-1277
Popis: Background Sexual transmission chains of Ebola virus (EBOV) have been verified and linked to EBOV RNA persistence in semen, post-recovery. The rate of semen persistence over time, including the average duration of persistence among Ebola virus disease (EVD) survivors, is not well known. This cohort study aimed to analyze population estimates of EBOV RNA persistence rates in semen over time, and associated risk factors in a population of survivors from Sierra Leone. Methods and findings In this cohort study from May 2015 to April 2017 in Sierra Leone, recruitment was conducted in 2 phases; the first enrolled 100 male participants from the Western Area District in the capital of Freetown, and the second enrolled 120 men from the Western Area District and from Lungi, Port Loko District. Mean age of participants was 31 years. The men provided semen for testing, analyzed by quantitative reverse transcription PCR (qRT-PCR) for the presence of EBOV RNA. Follow-up occurred every 2 weeks until the endpoint, defined as 2 consecutive negative qRT-PCR results of semen specimen testing for EBOV RNA. Participants were matched with the Sierra Leone EVD case database to retrieve cycle threshold (Ct) values from the qRT-PCR analysis done in blood during acute disease. A purposive sampling strategy was used, and the included sample composition was compared to the national EVD survivor database to understand deviations from the general male survivor population. At 180 days (6 months) after Ebola treatment unit (ETU) discharge, the EBOV RNA semen positive rate was 75.4% (95% CI 66.9%–82.0%). The median persistence duration was 204 days, with 50% of men having cleared their semen of EBOV RNA after this time. At 270 days, persistence was 26.8% (95% CI 20.0%–34.2%), and at 360 days, 6.0% (95% CI 3.1%–10.2%). Longer persistence was significantly associated with severe acute disease, with probability of persistence in this population at 1 year at 10.1% (95% CI 4.6%–19.8%) compared to the probability approaching 0% for those with mild acute disease. Age showed a dose–response pattern, where the youngest men (≤25 years) were 3.17 (95% CI 1.60, 6.29) times more likely to be EBOV RNA negative in semen, and men aged 26–35 years were 1.85 (95% CI 1.04, 3.28) times more likely to be negative, than men aged >35 years. Among participants with both severe acute EVD and a higher age (>35 years), persistence remained above 20% (95% CI 6.0%–50.6%) at 1 year. Uptake of safe sex recommendations 3 months after ETU discharge was low among a third of survivors. The sample was largely representative of male survivors in Sierra Leone. A limitation of this study is the lack of knowledge about infectiousness. Conclusions In this study we observed that EBOV RNA persistence in semen was a frequent phenomenon, with high population rates over time. This finding will inform forthcoming updated recommendations on risk reduction strategies relating to sexual transmission of EBOV. Our findings support implementation of a semen testing program as part of epidemic preparedness and response. Further, the results will enable planning of the magnitude of testing and targeted counseling needs over time.
Author summary Why was this study done? Evidence of traces of Ebola virus in semen had been reported among survivors for a very long time after their disease. This study originated out of a need to expand understanding beyond single reported cases, to gain knowledge on how many male survivors have Ebola virus persistence in semen over time, and for how long on average. We also aimed to analyze factors associated with longer persistence that could provide information on hypotheses on why some men carry virus in their semen for a very long time after the acute disease. What did the researchers do and find? In this study, 220 men who had survived Ebola disease in Sierra Leone during 2015–2016 provided semen specimens, and all of those who had traces of Ebola virus detected were followed every other week until their specimen turned negative. Seventy-five percent of these men still had traces of Ebola virus in their semen specimen at 6 months after being discharged following acute EVD, and 50% at 204 days. We also found that longer persistence of virus was significantly associated with severe acute EVD and older age. What do these findings mean? We show there is an urgent need to organize a national semen testing program as part of Ebola epidemic preparedness and response. The findings also show that in addition to testing, targeted safe sex counseling and free access to condoms should be a priority from the start of an outbreak. While we show that most of the survivors had traces of virus in semen at 6 months, more research is needed to understand the impact of viral RNA in semen on transmission of Ebola virus.
Databáze: OpenAIRE
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