Optimized Use of a 50 μm Internal Diameter Secondary Column in a Comprehensive Two-Dimensional Gas Chromatography System
| Autor: | Peter Quinto Tranchida, Giovanni Dugo, Luigi Mondello, Giorgia Purcaro, Lanfranco S. Conte, Paola Dugo |
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| Rok vydání: | 2009 |
| Předmět: |
Chromatography
Gas Capillary action Instrumentation Analytical chemistry Column combinations Comprehensive two-dimensional gas chromatography Flame ionization detectors Gas flows GC x GC High resolution Internal diameters Linear velocity Optimized use Separation power Optimized use Analytical Chemistry law.invention Internal diameters Separation power law Flame ionization detectors Column combinations Flame ionization detector High resolution Column (data store) Linear velocity Chromatography GC x GC Chemistry Gas flows Two-dimensional chromatography Comprehensive 2-D GC Polar Two-dimensional gas Gas chromatography Comprehensive two-dimensional gas chromatography |
| Zdroj: | Analytical Chemistry. 81:8529-8537 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/ac901461y |
| Popis: | The focus of the present research is directed toward the development of a comprehensive two-dimensional gas chromatography (GC x GC) method, characterized by a greatly increased separation power, if compared with GC x GC approaches using classical column combinations. The analytical objective was achieved by using a 0.05 mm internal diameter (i.d.) capillary as second dimension, a split-flow approach reported in previous research (Tranchida, P. Q., Casilli, A., Dugo, P., Dugo, G. and Mondello, L. Anal. Chem. 2007, 79, 2266-2275), and a twin-oven GC x GC instrument. The column combination employed was an orthogonal one: an apolar 30 m x 0.25 mm i.d. column was linked, by means of a Y-union, to a flame ionization detector (FID)-connected high-resolution 1 m x 0.05 mm i.d. polar one and to a 0.20 m x 0.05 mm i.d. uncoated capillary segment; the latter was connected to a manually operated split valve, located on top of the second GC. As previously shown, the generation of optimum gas linear velocities in both dimensions can be attained by splitting gas flows at the outlet of the first dimension (Tranchida, P. Q., Casilli, A., Dugo, P., Dugo, G. and Mondello, L. Anal. Chem. 2007, 79, 2266-2275). An optimized GC x GC method was developed and exploited for the analysis of a complex petrochemical sample. The satisfactory results attained were directly compared with those observed using the same instrumentation, equipped with what can be defined as a classical GC x GC split-flow column set: the same primary column was connected to an FID-linked 1 m x 0.10 mm i.d. polar one and to a 0.30 m x 0.10 mm i.d. uncoated capillary. It will be herein illustrated that there is still room for significant progress in the GC x GC field. |
| Databáze: | OpenAIRE |
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