Acute Exercise Improves Insulin Clearance and Increases the Expression of Insulin-Degrading Enzyme in the Liver and Skeletal Muscle of Swiss Mice
| Autor: | Hygor N. Araujo, Everardo M. Carneiro, Tarlliza R. Nardelli, Sandra Mara Ferreira, Antonio C. Boschero, Gustavo J. Santos, José M. Costa-Júnior, Luiz F. Rezende, Mirian Ayumi Kurauti, Jean Franciesco Vettorazzi, Ricardo Freitas-Dias |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Physiology medicine.medical_treatment lcsh:Medicine AMP-Activated Protein Kinases Insulysin Biochemistry Mice 0302 clinical medicine Endocrinology Blood plasma Insulin Secretion Insulin-degrading enzyme Medicine and Health Sciences Medicine Insulin lcsh:Science Musculoskeletal System Multidisciplinary Organic Compounds Hydrolysis Muscles Respiration Monosaccharides VO2 max Hep G2 Cells Hematology Body Fluids Chemistry medicine.anatomical_structure Blood Liver Physiological Parameters Physical Sciences Anatomy Research Article medicine.medical_specialty Carbohydrates 030209 endocrinology & metabolism Physical exercise Blood Plasma Cell Line 03 medical and health sciences Enzyme activator Islets of Langerhans Oxygen Consumption Internal medicine Physical Conditioning Animal Animals Humans Obesity Muscle Skeletal Diabetic Endocrinology Endocrine Physiology business.industry Organic Chemistry Body Weight lcsh:R Chemical Compounds Skeletal muscle Biology and Life Sciences Hormones Enzyme Activation 030104 developmental biology Glucose Skeletal Muscles Insulin Tolerance lcsh:Q business Physiological Processes |
| Zdroj: | PLoS ONE, Vol 11, Iss 7, p e0160239 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The effects of exercise on insulin clearance and IDE expression are not yet fully elucidated. Here, we have explored the effect of acute exercise on insulin clearance and IDE expression in lean mice. Male Swiss mice were subjected to a single bout of exercise on a speed/angle controlled treadmill for 3-h at approximately 60–70% of maximum oxygen consumption. As expected, acute exercise reduced glycemia and insulinemia, and increased insulin tolerance. The activity of AMPK-ACC, but not of IR-Akt, pathway was increased in the liver and skeletal muscle of trained mice. In an apparent contrast to the reduced insulinemia, glucose-stimulated insulin secretion was increased in isolated islets of these mice. However, insulin clearance was increased after acute exercise and was accompanied by increased expression of the insulin-degrading enzyme (IDE), in the liver and skeletal muscle. Finally, C2C12, but not HEPG2 cells, incubated at different concentrations of 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) for 3-h, showed increased expression of IDE. In conclusion, acute exercise increases insulin clearance, probably due to an augmentation of IDE expression in the liver and skeletal muscle. The elevated IDE expression, in the skeletal muscle, seems to be mediated by activation of AMPK-ACC pathway, in response to exercise. We believe that the increase in the IDE expression, comprise a safety measure to maintain glycemia at or close to physiological levels, turning physical exercise more effective and safe. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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