Axonal outgrowth stimulation after alginate/mesenchymal stem cell therapy in injured rat spinal cord

Autor: Eva Szekiova, Juraj Blasko, Lucia Slovinska, Jozef Kafka, Dasa Cizkova
Rok vydání: 2018
Předmět:
0301 basic medicine
Nervous system
Male
Pathology
medicine.medical_specialty
Alginates
Context (language use)
Biocompatible Materials
In Vitro Techniques
Mesenchymal Stem Cell Transplantation
Lesion
03 medical and health sciences
0302 clinical medicine
GAP-43 Protein
Glucuronic Acid
Spinal cord compression
Glial Fibrillary Acidic Protein
medicine
Animals
Organic Chemicals
Rats
Wistar
Spinal Cord Injuries
Analysis of Variance
business.industry
General Neuroscience
Hexuronic Acids
Mesenchymal stem cell
Mesenchymal Stem Cells
General Medicine
Spinal cord
medicine.disease
Flow Cytometry
Axons
Rats
Disease Models
Animal
030104 developmental biology
medicine.anatomical_structure
Exploratory Behavior
Cytokines
Bone marrow
Microglia
medicine.symptom
business
030217 neurology & neurosurgery
Psychomotor Performance
Astrocyte
Zdroj: Acta neurobiologiae experimentalis. 77(4)
ISSN: 1689-0035
Popis: Despite strong efforts in the field, spinal cord trauma still belongs among the untreatable neurological conditions at present. Given the complexity of the nervous system, an effective therapy leading to complete recovery has still not been found. One of the potential tools for supporting tissue regeneration may be found in mesenchymal stem cells, which possess anti‑inflammatory and trophic factor‑producing properties. In the context of transplantations, application of degradable biomaterials which could form a supportive environment and scaffold to bridge the lesion area represents another attractive strategy. In the present study, through a combination of these two approaches we applied both alginate hydrogel biomaterial alone or allogenic transplants of MSCs isolated from bone marrow seeded in alginate biomaterial into injured rat spinal cord at three weeks after spinal cord compression performed at Th8‑9 level. Following three‑week survival, using immunohistochemistry we studied axonal growth (GAP‑43 expression) and both microglia (Iba‑1) and astrocyte (GFAP) reactions at the lesion site and in the segments below and above the lesion. To detect functional improvement, during whole survival period we performed behavioral analyses of locomotor abilities using a classical open field test (BBB score) and a Catwalk automated gait analyzing device (Noldus). We found that despite the absence of locomotor improvement, application of both alginate and MSCs caused significant increase in the number of GAP‑43 positive axons.
Databáze: OpenAIRE
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