Translational repression by a transcriptional elongation factor
| Autor: | Luis Kameyama, Gabriel Guarneros, Helen R. Wilson, Donald L. Court, Jian-guang Zhou |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Gene Expression Regulation
Viral Genes Viral Molecular Sequence Data Repressor Biology Polymerase Chain Reaction Genes Reporter Transcription (biology) Gene expression Genetics Viral Regulatory and Accessory Proteins Transcription factor Gene Psychological repression DNA Primers Base Sequence Models Genetic Chromosome Mapping Bacteriophage lambda Protein Biosynthesis Antitermination Mutation Transcription preinitiation complex Nucleic Acid Conformation RNA Viral Transcription Factors Research Paper Developmental Biology |
| Zdroj: | Genes & Development. 11:2204-2213 |
| ISSN: | 1549-5477 0890-9369 |
| DOI: | 10.1101/gad.11.17.2204 |
| Popis: | One of the classical positive regulators of gene expression is bacteriophage λ N protein. N regulates the transcription of early phage genes by participating in the formation of a highly processive, terminator-resistant transcription complex and thereby stimulates the expression of genes lying downstream of transcriptional terminators. Also included in this antiterminating transcription complex are an RNA site (NUT) and host proteins (Nus). Here we demonstrate that N has an additional, hitherto unknown regulatory role, as a repressor of the translation of its own gene. N-dependent repression does not occur when NUT is deleted, demonstrating that N-mediated antitermination and translational repression both require the same cis-acting site in the RNA. In addition, we have identified one nut and several host mutations that eliminate antitermination and not translational repression, suggesting the independence of these two N-mediated mechanisms. Finally, the position of nutL with respect to the gene whose expression is repressed is important. |
| Databáze: | OpenAIRE |
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