High mobility group chromosomal protein 1 binds to the adeno-associated virus replication protein (Rep) and promotes Rep-mediated site-specific cleavage of DNA, ATPase activity and transcriptional repression
| Autor: | Lewis I. Pizer, Philippe Saudan, Peter Beard, Eithne Costello, Ernest Winocour |
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| Rok vydání: | 1997 |
| Předmět: |
Gene Expression Regulation
Viral Transcription Genetic Immunoprecipitation viruses Repressor Biology medicine.disease_cause DNA-binding protein General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Viral Proteins Transcription (biology) medicine Humans Binding site Molecular Biology Adeno-associated virus Adenosine Triphosphatases Binding Sites General Immunology and Microbiology General Neuroscience High Mobility Group Proteins DNA biochemical phenomena metabolism and nutrition Dependovirus Molecular biology DNA-Binding Proteins Repressor Proteins High-mobility group chemistry HeLa Cells Protein Binding Research Article |
| Zdroj: | The EMBO journal. 16(19) |
| ISSN: | 0261-4189 |
| Popis: | High mobility group protein 1 (HMG1) is an abundant non-histone chromosomal protein which plays a role in several nuclear events involving DNA. Here we demonstrate that HMG1 physically interacts with the human adeno-associated virus (AAV) Rep protein. HMG1 promotes the formation of Rep-DNA complexes and stimulates the activity of Rep in site- and strand-specific cleavage of DNA and the hydrolysis of ATP, functions required for viral gene regulation, replication and site-specific integration of viral DNA into human chromosome 19. We show that HMG1 enhances Rep-mediated repression of the AAV p5 promoter in transfected cells, suggesting that HMG1 and Rep also interact in vivo. HMG1, Rep and DNA can be immunoprecipitated as a ternary complex. Kinetic studies indicate that complexes of Rep with DNA have similar stabilities in the presence and absence of HMG1. These results suggest that the effect of HMG1 on Rep binding is exerted at the step of complex formation and thereby may reflect an activity of HMG1 in promoting the assembly of complex cellular nucleoprotein structures. |
| Databáze: | OpenAIRE |
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