CRISPR/Cas9 gene drives in genetically variable and nonrandomly mating wild populations
| Autor: | Amy L. Dapper, Douglas W. Drury, Michael J. Wade, Gabriel E. Zentner, Dylan J. Siniard |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
selfish gene population suppression inbreeding Population genetics Biology 03 medical and health sciences Genetic variation Animals CRISPR Genetic variability Allele CRISPR/Cas9 reproductive and urinary physiology Research Articles Genetics Tribolium Multidisciplinary Models Genetic Cas9 Reproduction fungi Genetic Variation SciAdv r-articles immune to drive food and beverages nonrandom mating Gene drive 030104 developmental biology population engineering behavior and behavior mechanisms gene drive CRISPR-Cas Systems Inbreeding Population Genetics Research Article |
| Zdroj: | Science Advances |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.1601910 |
| Popis: | Mating system and genetic variation impede the spread of gene drives, which target natural populations of disease-vectoring insects. Synthetic gene drives based on CRISPR/Cas9 have the potential to control, alter, or suppress populations of crop pests and disease vectors, but it is unclear how they will function in wild populations. Using genetic data from four populations of the flour beetle Tribolium castaneum, we show that most populations harbor genetic variants in Cas9 target sites, some of which would render them immune to drive (ITD). We show that even a rare ITD allele can reduce or eliminate the efficacy of a CRISPR/Cas9-based synthetic gene drive. This effect is equivalent to and accentuated by mild inbreeding, which is a characteristic of many disease-vectoring arthropods. We conclude that designing such drives will require characterization of genetic variability and the mating system within and among targeted populations. |
| Databáze: | OpenAIRE |
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