Xenobiotic Compounds Degradation by Heterologous Expression of a Trametes sanguineus Laccase in Trichoderma atroviride

Autor: Olivier Savary, Ulises Esquivel-Naranjo, Hubert Cabana, Vaidyanathan Vinoth Kumar, Marcela Ayala, Jorge Luis Folch-Mallol, Alfredo Herrera-Estrella, Luz Helena Méndez-Lorenzo, Edgar Balcázar-López, Ramón Alberto Batista-García
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Molecular biology
Applied Microbiology
lcsh:Medicine
Laccases
Biochemistry
Substrate Specificity
chemistry.chemical_compound
Trametes
Gene Expression Regulation
Fungal
Enzyme Stability
Post-Translational Modification
lcsh:Science
Thermostability
Trichoderma
Multidisciplinary
biology
Vector Construction
6. Clean water
Recombinant Proteins
Enzymes
Chemistry
Physical Sciences
Engineering and Technology
Signal Peptides
Bioremediation
Research Article
Biotechnology
Pollutants
Environmental Engineering
030106 microbiology
DNA construction
Microbiology
Xenobiotics
03 medical and health sciences
Environmental Biotechnology
Phenols
Genetics
Gene Expression and Vector Techniques
Environmental Chemistry
Laccase
Molecular Biology Assays and Analysis Techniques
Ecology and Environmental Sciences
lcsh:R
Chemical Compounds
Organisms
Fungi
Biology and Life Sciences
Proteins
biology.organism_classification
Pycnoporus
Enzyme Activation
Research and analysis methods
Kinetics
030104 developmental biology
Molecular biology techniques
chemistry
Genetic Loci
Enzymology
lcsh:Q
Heterologous expression
Xenobiotic
Zdroj: PLoS ONE, Vol 11, Iss 2, p e0147997 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: Fungal laccases are enzymes that have been studied because of their ability to decolorize and detoxify effluents; they are also used in paper bleaching, synthesis of polymers, bioremediation, etc. In this work we were able to express a laccase from Trametes (Pycnoporus) sanguineus in the filamentous fungus Trichoderma atroviride. For this purpose, a transformation vector was designed to integrate the gene of interest in an intergenic locus near the blu17 terminator region. Although monosporic selection was still necessary, stable integration at the desired locus was achieved. The native signal peptide from T. sanguineus laccase was successful to secrete the recombinant protein into the culture medium. The purified, heterologously expressed laccase maintained similar properties to those observed in the native enzyme (Km and kcat and kcat/km values for ABTS, thermostability, substrate range, pH optimum, etc). To determine the bioremediation potential of this modified strain, the laccase-overexpressing Trichoderma strain was used to remove xenobiotic compounds. Phenolic compounds present in industrial wastewater and bisphenol A (an endocrine disruptor) from the culture medium were more efficiently removed by this modified strain than with the wild type. In addition, the heterologously expressed laccase was able to decolorize different dyes as well as remove benzo[α]pyrene and phenanthrene in vitro, showing its potential for xenobiotic compound degradation.
Databáze: OpenAIRE
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