Antioxidant activities and molecular mechanisms of the ethanol extracts of Baccharis propolis and Eucalyptus propolis in RAW64.7 cells

Autor: Xiaoge Shen, Huoqing Zheng, Jiang-Lin Zhang, Xueping Cao, Kai Wang, Cui-Ping Zhang, Fuliang Hu
Rok vydání: 2016
Předmět:
0301 basic medicine
Antioxidant
Cell Survival
NF-E2-Related Factor 2
medicine.medical_treatment
Pharmaceutical Science
High-performance liquid chromatography
p38 Mitogen-Activated Protein Kinases
Antioxidants
Propolis
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Chlorogenic acid
Western blot
Phenols
Drug Discovery
medicine
Animals
Extracellular Signal-Regulated MAP Kinases
Chromatography
High Pressure Liquid
Pharmacology
Eucalyptus
Traditional medicine
medicine.diagnostic_test
biology
Dose-Response Relationship
Drug
Ethanol
Baccharis
Macrophages
General Medicine
biology.organism_classification
Oxidative Stress
030104 developmental biology
RAW 264.7 Cells
Complementary and alternative medicine
chemistry
Biochemistry
Gene Expression Regulation
030220 oncology & carcinogenesis
Kaempferide
Solvents
Molecular Medicine
Reactive Oxygen Species
Signal Transduction
Zdroj: Pharmaceutical biology. 54(10)
ISSN: 1744-5116
Popis: Context Numerous studies have reported that propolis possesses strong antioxidant activities. However, their antioxidant molecular mechanisms are unclear. Objective We utilize ethanol extracts of Chinese propolis (EECP) as a reference to compare ethanol extracts of Eucalyptus propolis (EEEP) with ethanol extracts of Baccharis propolis (EEBGP) based on their antioxidant capacities and underlying molecular mechanisms. Materials and methods HPLC and chemical analysis are utilized to evaluate compositions and antioxidant activities. ROS-eliminating effects of EEBGP (20-75 μg/mL), EEEP (1.25-3.75 μg/mL) and EECP (1.25-5 μg/mL) are also determined by flow cytometry analysis. Moreover, we compared antioxidant capacities by determining their effects on expressions of antioxidant genes in RAW264.7 cells with qRT-PCR, western blot and confocal microscopy analysis. Results EEBGP mainly contains chlorogenic acid (8.98 ± 0.86 mg/g), kaempferide (11.18 ± 8.31 mg/g) and artepillin C (107.70 ± 10.86 mg/g), but EEEP contains 10 compositions, whereas EECP contains 17 compositions. Meantime, although EEEP shows DPPH (IC50 19.55 ± 1.28), ABTS (IC50 20.0 ± 0.31) and reducing power (2.70 ± 0.08 mmol TE/g) better than EEBGP's DPPH (IC50 43.85 ± 0.54), ABTS (IC50 38.2 ± 0.33) and reducing power (1.53 ± 0.05 mmol TE/g), EEBGP exerts much higher ROS inhibition rate (40%) than EEEP (under 20%). Moreover, EEBGP strengthen antioxidant system by activating p38/p-p38 and Erk/p-Erk kinase via accelerating nucleus translocation of Nrf2. EEEP and EECP improve antioxidant gene expression only via Erk/p-Erk kinase-Nrf2 signalling pathway. Discussion and conclusion EEBGP and EEEP exert antioxidant activities via different molecular mechanisms, which may depend on chemical compositions.
Databáze: OpenAIRE
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