Differential effects of retinoids on pokeweed mitogen induced B-cell proliferation vs immunoglobulin synthesis
| Autor: | Xiao Ling Jiang, Eddie W. Lamon, Dirck L. Dillehay |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
Fenretinide Lipopolysaccharide Immunology Retinoic acid Immunoglobulins Tretinoin chemical and pharmacologic phenomena In Vitro Techniques Biology Lymphocyte Activation Mice Retinoids chemistry.chemical_compound Internal medicine medicine Splenocyte Animals Secretion Isotretinoin Pharmacology B-Lymphocytes Mice Inbred BALB C Cell growth Pokeweed mitogen Lymphokine Biological activity Kinetics Endocrinology Pokeweed Mitogens chemistry Female |
| Zdroj: | International Journal of Immunopharmacology. 13:1043-1048 |
| ISSN: | 0192-0561 |
| DOI: | 10.1016/0192-0561(91)90060-k |
| Popis: | The effects of three retinoids, all-trans-retinoic acid (RA), 13-cis-retinoic acid (cRA), and N-(4-hydroxyphenyl) retinamide (4-HPR) on mouse splenocyte responses to pokeweed mitogen (PWM) and Escherichia coli lipopolysaccharide (LPS) in vitro were evaluated. All three retinoids caused a dose dependent increase in the proliferative response to PWM. The retinoids hierarchy of efficacy based on potentiation of PWM-induced splenocyte proliferation was RA greater than cRA greater than 4-HPR. 13-cis-retinoic acid and 4-HPR also resulted in significant increases in Ig secretion in response to PWM. However, RA did not produce a significant increase in secretion compared with cells treated with PWM alone. The efficacy hierarchy of retinoids ability to potentiate Ig secretion was 4-HPR greater than cRA greater than RA. All three compounds did not affect Ig secretion from LPS-stimulated splenocytes and produced dose dependent decreases in proliferation. Both inhibition of LPS-induced proliferation and potentiation of PWM-induced proliferation were maximal when the retinoids were added during the first hour of culture. These results indicate that retinoids have a differential effect on Ig secretion and B-cell proliferation based on structural differences of the retinoids. Potentiation of proliferation and Ig secretion are both T-cell dependent and could be a result of increased lymphokine synthesis by the T-cells or increased responsiveness to the effects of the T-cell produced lymphokines. |
| Databáze: | OpenAIRE |
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