Fast methods of fungal and bacterial identification. MALDI-TOF mass spectrometry, chromogenic media
| Autor: | Fernando Sánchez-Juanes, María Siller-Ruiz, Juan Luis Muñoz-Bellido, José Manuel González-Buitrago, Sara Hernández-Egido |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Time Factors Microorganism 030106 microbiology Mass spectrometry Specimen Handling Microbiology 03 medical and health sciences Humans Mycological Typing Techniques Bacteria Staining and Labeling biology Chromogenic Fungi Bacterial Infections Chromogenic Compounds MALDI-TOF Mass Spectrometry biology.organism_classification Yeast Bacterial Typing Techniques Body Fluids Culture Media Mycoses Biochemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Identification (biology) |
| Zdroj: | Enfermedades infecciosas y microbiologia clinica (English ed.). 35:303-313 |
| ISSN: | 2529-993X |
| DOI: | 10.1016/j.eimce.2017.03.016 |
| Popis: | MALDI-TOF mass spectrometry is now a routine resource in Clinical Microbiology, because of its speed and reliability in the identification of microorganisms. Its performance in the identification of bacteria and yeasts is perfectly contrasted. The identification of mycobacteria and moulds is more complex, due to the heterogeneity of spectra within each species. The methodology is somewhat more complex, and expanding the size of species libraries, and the number of spectra of each species, will be crucial to achieve greater efficiency. Direct identification from blood cultures has been implemented, since its contribution to the management of severe patients is evident, but its application to other samples is more complex. Chromogenic media have also contributed to the rapid diagnosis in both bacteria and yeast, since they accelerate the diagnosis, facilitate the detection of mixed cultures and allow rapid diagnosis of resistant species. |
| Databáze: | OpenAIRE |
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