ACCERBATIN, a small molecule at the intersection of auxin and reactive oxygen species homeostasis with herbicidal properties

Autor: Sean R. Cutler, Yuming Hu, Dieter Buyst, Ann Cuypers, Kris Morreel, José C. Martins, Dominique Van Der Straeten, Petr Klíma, Dajo Smet, Klára Hoyerová, Thomas Depaepe, Jan Petrášek, Wout Boerjan, Filip Vandenbussche
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Auxin efflux
Physiology
Arabidopsis
Amino Acids
Cyclic
Gene Expression
Plant Science
ETHYLENE BIOSYNTHESIS
triple response
Quinolones
01 natural sciences
chemistry.chemical_compound
herbicide
ABIOTIC STRESS
Arabidopsis thaliana
Homeostasis
heterocyclic compounds
Abscisic acid
SEEDLINGS
chemistry.chemical_classification
reactive oxygen species
APICAL HOOK DEVELOPMENT
response
food and beverages
ROOT HAIR DEVELOPMENT
ABSCISIC-ACID
Research Papers
Cell biology
quinoline carboxamide
chemical genetics
Biochemistry
auxin homeostasis
ethylene signaling
Growth and Development
CELL ELONGATION
GENE FAMILY
Root hair
Biology
03 medical and health sciences
Auxin
Reactive oxygen species
SHOOT GRAVITROPISM
Auxin homeostasis
Indoleacetic Acids
Arabidopsis Proteins
Herbicides
triple
fungi
Biology and Life Sciences
Meristem
Ethylenes
biology.organism_classification
030104 developmental biology
chemistry
PLANT-GROWTH
Seedlings
ARABIDOPSIS-THALIANA
010606 plant biology & botany
Zdroj: Journal of Experimental Botany
JOURNAL OF EXPERIMENTAL BOTANY
ISSN: 1460-2431
0022-0957
Popis: ACCERBATIN is an ethylene-mimicking small molecule that affects auxin homeostasis and ROS accumulation in etiolated seedlings. In light-grown plants, it exhibits auxin-like herbicidal properties.
The volatile two-carbon hormone ethylene acts in concert with an array of signals to affect etiolated seedling development. From a chemical screen, we isolated a quinoline carboxamide designated ACCERBATIN (AEX) that exacerbates the 1-aminocyclopropane-1-carboxylic acid-induced triple response, typical for ethylene-treated seedlings in darkness. Phenotypic analyses revealed distinct AEX effects including inhibition of root hair development and shortening of the root meristem. Mutant analysis and reporter studies further suggested that AEX most probably acts in parallel to ethylene signaling. We demonstrated that AEX functions at the intersection of auxin metabolism and reactive oxygen species (ROS) homeostasis. AEX inhibited auxin efflux in BY-2 cells and promoted indole-3-acetic acid (IAA) oxidation in the shoot apical meristem and cotyledons of etiolated seedlings. Gene expression studies and superoxide/hydrogen peroxide staining further revealed that the disrupted auxin homeostasis was accompanied by oxidative stress. Interestingly, in light conditions, AEX exhibited properties reminiscent of the quinoline carboxylate-type auxin-like herbicides. We propose that AEX interferes with auxin transport from its major biosynthesis sites, either as a direct consequence of poor basipetal transport from the shoot meristematic region, or indirectly, through excessive IAA oxidation and ROS accumulation. Further investigation of AEX can provide new insights into the mechanisms connecting auxin and ROS homeostasis in plant development and provide useful tools to study auxin-type herbicides.
Databáze: OpenAIRE
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