No Evidence that Wnt Ligands Are Required for Planar Cell Polarity in Drosophila
| Autor: | Ben Ewen-Campen, Norbert Perrimon, Eric Vogt, Typhaine Comyn |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Frizzled animal structures Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine RNA interference WNT4 Animals Drosophila Proteins CRISPR Wnt Signaling Pathway chemistry.chemical_classification biology fungi Wnt signaling pathway Cell Polarity biology.organism_classification Notum Cell biology Dishevelled Wnt Proteins 030104 developmental biology chemistry Drosophila melanogaster 030217 neurology & neurosurgery |
| Zdroj: | Cell reports |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2020.108121 |
| Popis: | SUMMARY The frizzled (fz) and dishevelled (dsh) genes are highly conserved members of both the planar cell polarity (PCP) pathway and the Wnt signaling pathway. Given these dual functions, several studies have examined whether Wnt ligands provide a tissue-scale orientation cue for PCP establishment during development, and these studies have reached differing conclusions. Here, we re-examine this issue in the Drosophila melanogaster wing and notum using split-Gal4 co-expression analysis, multiplex somatic CRISPR, and double RNAi experiments. Pairwise loss-of-function experiments targeting wg together with other Wnt genes, via somatic CRISPR or RNAi, do not produce PCP defects in the wing or notum. In addition, somatic CRISPR against evi (aka wntless), which is required for the secretion of Wnt ligands, did not produce detectable PCP phenotypes. Altogether, our results do not support the hypothesis that Wnt ligands contribute to PCP signaling in the Drosophila wing or notum. Graphical Abstract In Brief Previous studies have come to differing conclusions on whether Wnt ligands provide a tissue-level orientation cue for the planar cell polarity pathway. Ewen-Campen et al. re-examine this question in Drosophila using multiplex in vivo CRISPR and double RNAi against Wnt ligands and find no evidence that Wnts are required for PCP patterning. |
| Databáze: | OpenAIRE |
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