Evolution and mutations predisposing to daptomycin resistance in vancomycin-resistant Enterococcus faecium ST736 strains

Autor: Abhay Dhand, Karthikeyan Murugesan, Fan Yu, Jian Zhuge, Weihua Huang, Leslie Lee, Henry Lin, Changhong Yin, Nevenka Dimitrova, Guiqing Wang, Andrew G. Hoss, John T. Fallon, Marisa A. Montecalvo
Rok vydání: 2018
Předmět:
Bacterial Diseases
0301 basic medicine
Nosocomial Infections
Gene Identification and Analysis
Drug resistance
Pathology and Laboratory Medicine
Medicine and Health Sciences
Evolutionary Emergence
Data Management
Antiinfective agent
education.field_of_study
Multidisciplinary
biology
Phylogenetic Analysis
Anti-Bacterial Agents
Bacterial Pathogens
Phylogenetics
Infectious Diseases
Medical Microbiology
Medicine
Pathogens
Research Article
medicine.drug
Computer and Information Sciences
Evolutionary Processes
Science
030106 microbiology
Population
Microbial Sensitivity Tests
Research and Analysis Methods
Microbiology
Vancomycin-Resistant Enterococci
Evolution
Molecular
03 medical and health sciences
Antibiotic resistance
Bacterial Proteins
Daptomycin
Microbial Control
Enterococcus Infections
Genetics
medicine
Humans
Evolutionary Systematics
Molecular Biology Techniques
education
Mutation Detection
Microbial Pathogens
Molecular Biology
Gram-Positive Bacterial Infections
Taxonomy
Pharmacology
Evolutionary Biology
Whole Genome Sequencing
Bacteria
Organisms
Biology and Life Sciences
biology.organism_classification
Enterococcus
Mutation
Multilocus sequence typing
Antimicrobial Resistance
Genome
Bacterial
Multilocus Sequence Typing
Cloning
Enterococcus faecium
Zdroj: PLoS ONE
PLoS ONE, Vol 13, Iss 12, p e0209785 (2018)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0209785
Popis: We recently identified a novel vancomycin-resistant Enterococcus faecium (VREfm) clone ST736 with reduced daptomycin susceptibility. The objectives of this study were to assess the population dynamics of local VREfm strains and genetic alterations predisposing to daptomycin resistance in VREfm ST736 strains. Multilocus sequence typing and single nucleotide variant data were derived from whole-genome sequencing of 250 E. faecium isolates from 1994-1995 (n = 43), 2009-2012 (n = 115) and 2013 (n = 92). A remarkable change was noticed in the clonality and antimicrobial resistance profiles of E. faecium strains between 1994-1995 and 2013. VREfm sequence type 17 (ST17), the prototype strain of clade A1, was the dominant clone (76.7%) recognized in 1994-1995. By contrast, clone ST736 accounted for 46.7% of VREfm isolates, followed by ST18 (26.1%) and ST412 (20.7%) in 2013. Bayesian evolutionary analysis suggested that clone ST736 emerged between 1996 and 2009. Co-mutations (liaR.W73C and liaS.T120A) of the liaFSR system were identified in all ST736 isolates (n = 111, 100%) examined. Thirty-eight (34.2%) ST736 isolates exhibited daptomycin-resistant phenotype, of which 13 isolates had mutations in both the liaFSR and cardiolipin synthase (cls) genes and showed high level of resistance with a daptomycin MIC50 of 32 μg/mL. The emergence of ST736 strains with mutations predisposing to daptomycin resistance and subsequent clonal spread among inpatients contributed to the observed high occurrence of daptomycin resistance in VREfm at our institution. The expanding geographic distribution of ST736 strains in other states and countries raises concerns about its global dissemination.
Databáze: OpenAIRE
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