Synthesis and stability of retinal photoreceptor mRNAs are coordinately regulated during bovine fetal development
| Autor: | Adrian M. Timmers, Bruce R. Newton, William W. Hauswirth |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Rhodopsin
genetic structures Transcription Genetic Gestational Age Retina Cellular and Molecular Neuroscience Embryonic and Fetal Development Fetus Transcription (biology) 3' 5'-Cyclic-GMP Phosphodiesterases medicine Arrestin Animals Photoreceptor Cells RNA Messenger Transducin Antigens Eye Proteins Gene Regulation of gene expression Messenger RNA biology Membrane Proteins Blotting Northern Molecular biology Sensory Systems Retinol-Binding Proteins Ophthalmology medicine.anatomical_structure Gene Expression Regulation biology.protein Cattle sense organs |
| Zdroj: | Experimental eye research. 56(3) |
| ISSN: | 0014-4835 |
| Popis: | Steady state photoreceptor specific mRNA levels in bovine retina were studied during fetal maturation for five gene transcripts, including rhodopsin, arrestin (S-antigen), rod alpha-subunit of transducin, interphotoreceptor retinoid-binding protein (IRBP) and rod alpha-subunit of cGMP phosphodiesterase in order to understand mechanisms of gene regulation during photoreceptor development. A 10-15-fold increase in each transcript level begins between 5.5 and 6 months of gestation for each gene, suggesting a single coordinate induction event at this time. Quantitative analysis of transcriptional rates for each gene by nuclear run-on also reveals a coordinate increase at approximately the same time, demonstrating that induction is achieved by transcriptional activation. Interestingly, however, gene specific transcription rates in the pre-induction retina do not appear to parallel mRNA steady state levels. During fetal development neither the transcript level of each gene relative to the others nor relative mRNA turnover rates change substantially after the induction event at 5.5-6.0 months. However, at earlier times all genes exhibit higher mRNA turnover, implying that differential mRNA stability may also play an important role in determining steady-state levels. |
| Databáze: | OpenAIRE |
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