Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification
| Autor: | Barbara Bykowska, Natalia Kobylak, Anna Brillowska-Dąbrowska, Roman Nowicki |
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| Rok vydání: | 2014 |
| Předmět: |
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biology Arthrodermataceae Trichophyton rubrum MOLECULAR BIOLOGY METHODS bacterial infections and mycoses medicine.disease_cause biology.organism_classification Real-Time Polymerase Chain Reaction DNA extraction General Biochemistry Genetics and Molecular Biology Microbiology Real-time polymerase chain reaction Trichophyton Dermatophyte medicine Identification (biology) skin and connective tissue diseases |
| Zdroj: | Acta biochimica Polonica. 62(1) |
| ISSN: | 1734-154X |
| Popis: | Dermatophytes are keratinophilic molds that infect human hair, nails and skin. Diagnosis of dermatophytosis is based on morphological, serological and biochemical features. However, identification is difficult and laborious due to similarities between microorganisms. Thus, there is considerable interest to develop mycological diagnostic procedures based on molecular biology methods. In this study, fast, two-step DNA extraction method and real-time PCR was used for detection of dermatophytes DNA using pan-dermatophyte primers and identification of Trichophyton rubrum from pure cultures. The applied method allowed correct detection of all dermatophytes and correct identification of Trichophyton rubrum in less than 2 hours. |
| Databáze: | OpenAIRE |
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