Pseudomonas wadenswilerensis sp. nov. and Pseudomonas reidholzensis sp. nov. : two new species within the Pseudomonas putida group isolated from forest soil
Autor: | Tanja Torossi, Tara Picozzi, Michael Opoku, Urs Hilber, Theo H. M. Smits, Stefanie Balada, David Frasson |
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Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
DNA Bacterial Sequence analysis Ubiquinone Biology Forests Microbiology 03 medical and health sciences RNA Ribosomal 16S Pseudomonas Ecology Evolution Behavior and Systematics Phylogeny Soil Microbiology Base Composition Strain (chemistry) Pseudomonas putida DNA–DNA hybridization Phosphatidylethanolamines Biotechnological application Fatty Acids Nucleic Acid Hybridization General Medicine Sequence Analysis DNA Ribosomal RNA 570: Biologie biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology Screening Multilocus sequence typing Switzerland Multilocus Sequence Typing |
Popis: | Within the frame of a biotechnological screening, we isolated two Pseudomonas strains from forest soil. 16S rRNA gene sequence analysis indicated that strain CCOS 864T shared 99.8 % similarity with Pseudomonas donghuensis HYST, while strain CCOS 865T shared 99.0 % similarity with Pseudomonas putida DSM 291T and lower similarity with other P. putida group type strains. Based on multilocus sequence analysis, the two strains were genotypically distinct from each other, each forming a separate clade. Strains CCOS 864T and CCOS 865T were Gram-stain-negative, motile and rod-shaped, growing at a temperature range of 4–37 °C. Strain CCOS 864T could be phenotypically distinguished from P. putida group species by the combination of gelatinase-positive reaction and positive growth on N-acetyl-d-glucosamine, p-hydroxyphenylacetic acid and inosine but lack of fluorescein production on King’s B medium, while strain CCOS 865T could be distinguished from P. putida group species by the combination of positive growth with saccharic acid and negative growth with p-hydroxyphenylacetic acid and l-pyroglutamic acid. The major polar lipid for both strains was phosphatidylethanolamine; the major quinone was ubiquinone Q-9. DNA–DNA hybridization and average nucleotide identities confirmed the novel species status for the two strains. The DNA G+C contents of CCOS 864T and CCOS 865T were 62.1 and 63.8 mol%, respectively. The phenotypic, phylogenetic and DNA–DNA relatedness data support the suggestion that CCOS 864T and CCOS 865T represent two novel Pseudomonas species. The names Pseudomonas wadenswilerensis sp. nov. (type strain CCOS 864T=LMG 29327T) and Pseudomonas reidholzensis sp. nov. (type strain CCOS 865T=LMG 29328T) are proposed. |
Databáze: | OpenAIRE |
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