First detection of influenza A virus genes from wild raccoons in Japan
| Autor: | Emi Yamaguchi, Haruko Ogawa, Kei Fujii, Kunitoshi Imai |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty 030106 microbiology Sequence Homology Biology medicine.disease_cause Serology Viral Matrix Proteins 03 medical and health sciences Medical microbiology Japan Phylogenetics Virology parasitic diseases Genetics Influenza A virus medicine Animals Cluster Analysis Juvenile Molecular Biology Gene Phylogeny Viral matrix protein Reverse Transcriptase Polymerase Chain Reaction Viral Core Proteins Rectum RNA-Binding Proteins Sequence Analysis DNA General Medicine Nucleocapsid Proteins Nucleoprotein 030104 developmental biology Raccoons Nasal Cavity |
| Zdroj: | Virus Genes. 54:591-595 |
| ISSN: | 1572-994X 0920-8569 |
| DOI: | 10.1007/s11262-018-1566-z |
| Popis: | Serological surveys have shown that wild raccoons are exposed to influenza A viruses (IAVs); however, no genetic evidence for this IAV infection has been found. In the present study, we first detected IAV genes in wild raccoons captured during periods other than the wintering season of migratory waterfowl and epidemic season of influenza in Japan. Viral matrix (M) and nucleoprotein (NP) genes were detected by a conventional reverse transcription-polymerase chain reaction assay from three suckling siblings and one juvenile without any noticeable clinical signs, although the NP gene could not be detected from one sibling. The sequences of M gene fragments detected from the rectal swabs of three suckling siblings were comparable with each other but different from those detected from the nasal swab of the juvenile raccoon caught from a different site. The sequences of NP gene fragments detected from two suckling siblings were also comparable. These genetic evidences suggest that IAV is maintained among raccoon populations in the northern part of Japan. Further genetic and virological investigation of IAV infection in wild raccoons is needed to better understand the IAV ecology in the field. |
| Databáze: | OpenAIRE |
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