Whole genome analysis of a novel picornavirus related to the Enterovirus/Sapelovirus supergroup from porcine feces in Japan
| Autor: | Tsuneyuki Masuda, Tetsuya Mizutani, Tsutomu Omatsu, Fujiko Sunaga, Hiroshi Yamasato, Mami Oba, Yukie Katayama, Shoichi Sakaguchi, Mika Ito, Yuki Naoi, Hiroki Takagi, Makoto Nagai, Tetsuya Furuya, Shinji Makino, Junsuke Shirai |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Diarrhea Cancer Research food.ingredient Picornavirus Swine Genome Viral Picornaviridae medicine.disease_cause Virus 03 medical and health sciences Feces Open Reading Frames Viral Proteins food Japan Virology medicine Coding region Animals 3' Untranslated Regions Phylogeny Enterovirus Picornaviridae Infections biology Sequence Analysis DNA Ribosomal RNA biology.organism_classification Open reading frame 030104 developmental biology Infectious Diseases Novel virus Nucleic Acid Conformation RNA Viral Metagenomics 5' Untranslated Regions Sapelovirus |
| Zdroj: | Virus research. 257 |
| ISSN: | 1872-7492 |
| Popis: | A novel virus related to the Enterovirus/Sapelovirus supergroup in the family Picornaviridae was identified in healthy porcine feces in Japan by using a metagenomics approach. The genome of the virus, named Sapelo-like porcine picornavirus Japan (SPPVJ) Pig/Isi-Im1/JPN/2016, had a type-IV internal ribosomal entry site and carried a 6978-nucleotide-long single open reading frame encoding a 2326 amino acids (aa) polyprotein precursor. The coding sequence region consisted of leader protein (68 aa), a structural protein region P1 (824 aa), and the non-structural protein regions P2 (672 aa) and P3 (762 aa). Among representative picornaviruses, the P1, 2C, and 3CD regions of SPPVJ had the highest aa identities of 64.4%, 61.9%, and 73.3%, respectively, with the corresponding regions of sapelo-like bat picornavirus BtVs-PicoV/SC2013. Sequencing analysis of the RT-PCR products derived from the 5' untranslated and 3D regions revealed the presence of SPPVJ in 17.8% (19/107) of the feces from healthy and diarrheal pigs in 12 farms in 2015-2016. Further studies are needed to determine the origin and pathogenic potential of SPPJV in pigs and other mammals. |
| Databáze: | OpenAIRE |
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