An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding
| Autor: | Mee H. Hoe, Mark Stamnes, Scott J. Geromanos, Nina Lampen, Mark W. Craighead, Paul Tempst, James E. Rothman |
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| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Vesicle fusion
Protein family Molecular Sequence Data Arabidopsis Coated vesicle Golgi Apparatus CHO Cells Saccharomyces cerevisiae Biology Coatomer Protein Membrane Fusion Polymerase Chain Reaction Cytosol Dogs GTP-Binding Proteins Cricetinae Animals Humans Amino Acid Sequence Integral membrane protein DNA Primers Organelles Multidisciplinary Base Sequence Sequence Homology Amino Acid ADP-Ribosylation Factors Vesicle Brain Membrane Proteins Transport protein Cell biology Microscopy Electron Biochemistry Membrane protein Coatomer Cattle Carrier Proteins Research Article |
| Popis: | We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad repeats found in coiled coils. When the yeast p24 isoform, yp24A, is knocked out in a strain defective for vesicle fusion, a dramatic reduction in the accumulation of transport vesicles is observed. Together, these results indicate a role for this protein family in the budding of coatamer-coated and other species of coated vesicles. |
| Databáze: | OpenAIRE |
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