Phenotypic and functional immaturity of human umbilical cord blood T lymphocytes
| Autor: | Dominick DeLuca, David T. Harris, Judith Bard, Jeffrey Locascio, George B. Olson, Lewis Shenker, Michael J. Schumacher, Francoise J. Besencon, Edward A. Boyse |
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| Rok vydání: | 1992 |
| Předmět: |
Cytotoxicity
Immunologic T-Lymphocytes Placenta cord banking Biology Polymerase Chain Reaction Umbilical cord Cell Line Immunophenotyping Antigens CD medicine Animals Humans RNA Messenger HLA-D Antigens Lymphokines Multidisciplinary DNA Fetal Blood Flow Cytometry Killer Cells Natural Transplantation Blotting Southern Haematopoiesis medicine.anatomical_structure Cord blood Immunology Bone marrow Lymphocyte Culture Test Mixed Stem cell Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 89:10006-10010 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.89.21.10006 |
| Popis: | Successful implementation of bone marrow transplantation for hematopoietic reconstitution is limited by the lack of suitably HLA-matched donors and by the occurrence of graft-versus-host disease that frequently accompanies this procedure. Recent clinical reports have implied that the use of umbilical cord blood as a source of transplantable stem cells may solve these problems. To date, definitive experiments have not been performed to assess the immunological potential of T cells found in umbilical cord blood, which could mediate graft-versus-host disease. In the present study we have observed that umbilical cord blood contains T lymphocytes that appear to be phenotypically immature. In addition, umbilical cord blood lymphocytes appeared to be functionally immature as shown by minimal responses to stimulation with interleukin 2, phytohemagglutinin, or alloantigens. Thus, umbilical cord blood may be more suitable for allogeneic transplantation than bone marrow in that these cord blood cells may not be as capable of mediating graft-versus-host disease. |
| Databáze: | OpenAIRE |
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