Peripheral T-cell tolerance induced in naive and primed mice by subcutaneous injection of peptides from the major cat allergen Fel d I
| Autor: | Thomas J. Briner, Bruce L. Rogers, Kathleen M. Keating, Kuo Mei-Chang, Julia L. Greenstein |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
Interleukin 2
Cellular immunity T cell T-Lymphocytes Enzyme-Linked Immunosorbent Assay Mice Inbred Strains Biology Epitope Immunoglobulin G Immune tolerance Cell Line Interferon-gamma Mice Immune system medicine Animals Interferon gamma Cells Cultured Glycoproteins Immunosuppression Therapy Multidisciplinary Allergens Molecular biology medicine.anatomical_structure Immunology biology.protein Cats Interleukin-2 Biological Assay Female Interleukin-4 Lymph Nodes Spleen medicine.drug Research Article |
| Popis: | T cells control the majority of antigen-specific immune responses. Therefore, influencing the activation of the T-cell response in order to modify immune responsiveness is an obvious therapeutic goal. We have used a mouse model of response to Fel d I, the major cat protein allergen in humans, to explore the ability of peptides derived from Fel d I to inhibit T-cell-dependent immune responses to the peptides themselves and to larger polypeptides. T cells from B6CBAF1 mice respond to the Fel d I peptide IPC-2 after challenge with IPC-2. However, subcutaneous tolerization with IPC-2 prevents this response as measured by production of interleukins 2 and 4 and interferon gamma. Fel d I immunization of B6D2F1 mice results in T-cell responses primarily to one peptide derived from Fel d I. Injecting this peptide in soluble form inhibits T-cell activation (as measured by interleukin 2 production) and antibody production in Fel d I-primed animals when they are subsequently challenged with peptide in adjuvant. Most of the cat-allergic human T-cell response to Fel d I is specific for two peptides on one of its two chains. Immunization of B6CBAF1 mice with recombinant Fel d I chain 1 results in T-cell responses to the same peptides. Subcutaneous administration of these two peptides, which contain some, but not all, of the T-cell epitopes from Fel d I chain I, decreases the T-cell response to the entire recombinant Fel d I chain 1. The ability to tolerize T-cell responses with subcutaneous injections suggests a practical approach to treating human diseases with peptides containing T-cell epitopes. |
| Databáze: | OpenAIRE |
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