Evaluation of the Chagas Western Blot IgG Assay for the Diagnosis of Chagas Disease
| Autor: | S. Jauréguiberry, Margarita Bisio, Dominique Mazier, Ballering Griselda, Luc Paris, Julián Ernesto Nicolás Gulin, Jaime Altcheh, Jean-Yves Brossas, François-Xavier Lescure, Jeremy Guihenneuc, Arnaud Fekkar |
|---|---|
| Přispěvatelé: | Service de parasitologie - mycologie [CHU Pitié-Salpétrière], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Centre d'Immunologie et de Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Service des maladies infectieuses et tropicales [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Services de Maladies Infectieuses et Tropicales [CHU Bichat], AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Chagas disease [SDV.BIO]Life Sciences [q-bio]/Biotechnology Trypanosoma cruzi 030231 tropical medicine diagnostic Article Serology 03 medical and health sciences 0302 clinical medicine Western blot Antigen [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases parasitic diseases medicine Immunology and Allergy [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology Molecular Biology 0303 health sciences General Immunology and Microbiology biology medicine.diagnostic_test 030306 microbiology business.industry biology.organism_classification medicine.disease Virology 3. Good health Blot Infectious Diseases Parasitology biology.protein Medicine Antibody business |
| Zdroj: | Pathogens Pathogens, MDPI, 2021, 10 (11), pp.1455. ⟨10.3390/pathogens10111455⟩ Volume 10 Issue 11 Pathogens, Vol 10, Iss 1455, p 1455 (2021) |
| ISSN: | 2076-0817 |
| DOI: | 10.3390/pathogens10111455⟩ |
| Popis: | Chagas disease is a debilitating and often fatal pathology resulting from infection by the protozoan parasite Trypanosoma cruzi. In its recommendations, the World Health Organization states that the diagnosis of T. cruzi infection is usually based on the detection of antibodies against T. cruzi antigens and performed with two methodologically different assays. An inconclusive result can be resolved with a third “confirmatory” assay. The objective of this article is to evaluate the effectiveness of the Chagas Western Blot IgG assay (LDBio Diagnostics, Lyon, France) as a confirmatory serologic test. The Chagas Western Blot IgG assay was performed with native antigens derived from a T. cruzi strain of the TcVI genotype. Retrospective sera were provided by two parasitology laboratories (France and Argentina). The sensitivity, specificity, positive predictive value and negative predictive value of the Chagas blot were all 100% in our sera collection. The Chagas blot is an easy and qualitative method for the diagnosis of Chagas disease, with results in less than 2 h. This immunoblot has potential as a supplemental test for the confirmation of the presence of antibodies against T. cruzi in serum specimens. Nonetheless, the very good initial results presented here will need to be confirmed in larger studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|