Ultrasonically enhanced extraction of luteolin and apigenin from the leaves of Perilla frutescens (L.) Britt. using liquid carbon dioxide and ethanol
| Autor: | Hiroyuki Kawano, Takafumi Kato, Ryo Kawakami, Hirofumi Kawamura, Kenji Mishima, Hilyatuz Zahroh, Shota Ito, Fauziyah Hasanah, Takunori Harada, Makoto Misumi, Salim Mustofa, Yusraini Dian Inayati Siregar, Tadashi Suetsugu, Keiichi Irie, Lily Surayya Eka Putri, Tanjina Sharmin, Hideaki Orii, Kazunori Sano, Kenichi Mishima, Agus Salim |
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| Rok vydání: | 2016 |
| Předmět: |
Time Factors
Acoustics and Ultrasonics 02 engineering and technology Chemical Fractionation 010402 general chemistry 01 natural sciences High-performance liquid chromatography Inorganic Chemistry chemistry.chemical_compound Pressure Chemical Engineering (miscellaneous) Environmental Chemistry Radiology Nuclear Medicine and imaging Apigenin Luteolin Perilla frutescens Chromatography Ethanol biology Organic Chemistry Extraction (chemistry) Temperature Carbon Dioxide 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Plant Leaves Ultrasonic Waves chemistry Yield (chemistry) Solvents Particle size 0210 nano-technology |
| Zdroj: | Ultrasonics Sonochemistry. 29:19-26 |
| ISSN: | 1350-4177 |
| DOI: | 10.1016/j.ultsonch.2015.08.016 |
| Popis: | The present study reports on the ultrasonic enhancement of the liquid carbon dioxide (CO2) extraction of luteolin and apigenin from the leaves of Perilla frutescens (L.) Britt., to which ethanol is added as a cosolvent. The purpose of this research is also to investigate the effects of the particle size, temperature, pressure, irradiation power, irradiation time, and ethanol content in the liquid CO2 solution on the extraction yield using single-factor experiments. We qualitatively and quantitatively analyzed the yields in the extract using HPLC (high-performance liquid chromatography). The liquid CO2 mixed with ethanol was used at temperatures of 5, 20 and 25 °C with extraction pressures from 8 to 14 MPa. The yields of luteolin and apigenin in the extraction were clearly enhanced by the ultrasound irradiation, but the selectivity of the extract was not changed. The yields of luteolin and apigenin in the extract were also significantly improved by adjusting the operating temperature, the irradiation time, and the ethanol content in the liquid CO2 solution, but no change in the selectivity of the extract was observed. |
| Databáze: | OpenAIRE |
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