Age-dependent modulation of neuropeptide y on adrenergic transmission of guinea pig vas deferens
| Autor: | Maya Yankova, Lilia Chernaeva |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Aging medicine.medical_specialty Guinea Pigs Neuropeptide Adrenergic Stimulation In Vitro Techniques Peptide hormone Biology Synaptic Transmission Guinea pig Norepinephrine Vas Deferens Internal medicine mental disorders medicine Animals Neuropeptide Y Pharmacology Vas deferens Neuropeptide Y receptor Electric Stimulation medicine.anatomical_structure Endocrinology medicine.symptom Adrenergic Fibers Muscle Contraction Muscle contraction |
| Zdroj: | General Pharmacology: The Vascular System. 22:611-616 |
| ISSN: | 0306-3623 |
| DOI: | 10.1016/0306-3623(91)90065-e |
| Popis: | 1. The effect of neuropeptide (NPY) on [3H]noradrenaline ([3H]NA) release- and on contractions evoked by field electrical stimulation (FES) was studied in vitro in vas deferens from mature and immature guinea pigs. 2. The evoked tritium overflow (which reflected [3H]NA release) was determined by liquid scintillation spectrometry. 3. Field electrical stimulation of 5 Hz (trains of 50 pulses in 20 sec intervals) evoked guanethidine-sensitive contractions. 4. NPY (0.01-1 microM) dose-dependently inhibited the evoked contractions in both groups of animals. NPY, 1 microM, almost completely inhibited the evoked contractions in mature animals, while those in immature guinea pigs were inhibited but only by 80.4 +/- 3.6%. 5. The amount of tritium overflow evoked by 5 Hz stimulation (300 pulses: 15 trains of 20 pulses in 20 sec intervals) was higher in immature guinea pigs (0.46 +/- 0.03%) compared with the amount of the evoked tritium overflow in mature guinea pigs (0.39 +/- 0.02%). 6. NPY, 1 microM, inhibited the evoked tritium overflow. The NPY inhibition was more pronounced in vas deferens of mature (45.3 +/- 2.0%) than in immature (25.1 +/- 3.5%) guinea pigs. 7. The results suggest that NPY modulation of adrenergic transmission at the prejunctional level increases with the maturity. |
| Databáze: | OpenAIRE |
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