Neuropeptide Y (NPY) in Human Nasal Mucosa
| Autor: | Joaquim Mullol, Marco Merida, Sharon Castellino, Julie Goff, James H. Shelhamer, Michael A. Kaliner, Jens D Lundgren, James N. Baraniuk |
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| Rok vydání: | 1990 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Vascular smooth muscle Clinical Biochemistry Radioimmunoassay Mucous membrane of nose Biology Veins chemistry.chemical_compound Nerve Fibers Culture Techniques Internal medicine mental disorders medicine Humans Neuropeptide Y Molecular Biology Chromatography High Pressure Liquid Neurons Plexus Binding Sites Phosphoramidon Arteries Cell Biology Thiorphan Neuropeptide Y receptor Immunohistochemistry humanities Epithelium Kinetics Lactoferrin Nasal Mucosa medicine.anatomical_structure Endocrinology chemistry Autoradiography Glycoconjugates |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 3:165-173 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/ajrcmb/3.2.165 |
| Popis: | Neuropeptide Y (NPY), a potent vasoconstrictor peptide found in sympathetic neurons, was analyzed in human inferior turbinate nasal mucosal tissue. NPY content determined by radioimmunoassay was 3.13 +/- 0.79 pmol/g tissue (n = 6) in mucosa extracted with ethanol-acetic acid. NPY-immunoreactive nerves were found around small muscular arteries, arterioles, arteriovenous anastomoses, and as free fibers near arteriolar and venous vessels. They formed a plexus around the arterial vessels, and were also present between vascular smooth muscle cells. Few NPY fibers were present near glands or the epithelium. [125I]NPY binding sites were localized by autoradiography to small muscular arteries, arterioles, and a few venous sinusoids. In explant culture experiments, 4 microM NPY did not stimulate release of [3H]glucosamine-labeled glycoconjugates or lactoferrin (a product of serous cells) from nasal mucosal fragments. Degradation of NPY by a tissue homogenate was rapid (t1/2 = 13.5 +/- 2.3 min). The degradation was inhibited by thiorphan and phosphoramidon, inhibitors of neutral endopeptidase activity. NPY released from sympathetic neurons may play a role as a constrictor of arterial vessels and regulate vasomotor tone in the human nasal mucosa. |
| Databáze: | OpenAIRE |
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