Glycosidases in porcine follicular fluid and their effect on zona pellucida-AWN 1 spermadhesin interaction
| Autor: | Jiří Liberda, Pavla Maňásková-Postlerová, Marie Tichá, Štěpán Ren, Tomáš Dráb, Věra Jonáková |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
endocrine system Glycoside Hydrolases Swine Protein Array Analysis Biology Andrology 03 medical and health sciences Follicle Human fertilization Food Animals medicine Animals Biotinylation Small Animals Zona pellucida education Zona Pellucida chemistry.chemical_classification education.field_of_study Alpha-galactosidase urogenital system Equine Seminal Plasma Proteins Sperm receptor Oligosaccharide Oocyte Follicular fluid Follicular Fluid 030104 developmental biology medicine.anatomical_structure chemistry Biochemistry Oocytes biology.protein Female Animal Science and Zoology |
| Zdroj: | Theriogenology. 100:80-87 |
| ISSN: | 0093-691X |
| Popis: | Oligosaccharide moieties on the surface of the oocyte belong to the key molecules that direct the course of fertilization and are subjected to changes during oocyte maturation in the follicle. In our study, we focused on the activities of five glycosidases in the fluids from porcine secondary and preovulatory follicles (α-l-fucosidase, α-d-galactosidase, β-d-galactosidase, β-D-N-acetylhexosaminidase, and α-d-mannosidase). All of them were detected active at neutral and acidic pH. However, changes in their activities associated with follicle development were observed only in the case of α-d-mannosidase, which was increased (P |
| Databáze: | OpenAIRE |
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