Regulation of kinesin-1 activity by the Salmonella enterica effectors PipB2 and SifA
| Autor: | Patricia Bassereau, Alexandra Vergnes, Yaya Zhao, Dumizulu L. Tembo, Lorena Redondo-Morata, Stéphane Méresse, Margaux Lagier, Ziyan Fang, Nina Schroeder, Audrey Dumont, Lucrecia Alberdi, Jean-Pierre Gorvel, Jean-Baptiste Manneville |
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| Přispěvatelé: | Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2020 |
| Předmět: |
Salmonella
[SDV]Life Sciences [q-bio] macromolecular substances [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] [SDV.BC]Life Sciences [q-bio]/Cellular Biology Vacuole medicine.disease_cause 03 medical and health sciences 0302 clinical medicine [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] Molecular motor medicine Secretion ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Effector [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology biology.organism_classification [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Cell biology [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Salmonella enterica [SDV.IMM]Life Sciences [q-bio]/Immunology Kinesin [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] 030217 neurology & neurosurgery Intracellular |
| Zdroj: | Journal of Cell Science Journal of Cell Science, Company of Biologists, 2020, 133 (9), pp.jcs239863. ⟨10.1242/jcs.239863⟩ |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.239863 |
| Popis: | Salmonella enterica is an intracellular bacterial pathogen. The formation of its replication niche, which is composed of a vacuole associated with a network of membrane tubules, depends on the secretion of a set of bacterial effector proteins whose activities deeply modify the functions of the eukaryotic host cell. By recruiting and regulating the activity of the kinesin-1 molecular motor, Salmonella effectors PipB2 and SifA play an essential role in the formation of the bacterial compartments. In particular, they allow the formation of tubules from the vacuole and their extension along the microtubule cytoskeleton, and thus promote membrane exchanges and nutrient supply. We have developed in vitro and in cellulo assays to better understand the specific role played by these two effectors in the recruitment and regulation of kinesin-1. Our results reveal a specific interaction between the two effectors and indicate that, contrary to what studies on infected cells suggested, interaction with PipB2 is sufficient to relieve the autoinhibition of kinesin-1. Finally, they suggest the involvement of other Salmonella effectors in the control of the activity of this molecular motor. This article has an associated First Person interview with the first author of the paper. |
| Databáze: | OpenAIRE |
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