A triple co-culture model of the human respiratory tract to study immune-modulatory effects of liposomes and virosomes
| Autor: | Christophe von Garnier, Sean Hall, Fabian Blank, Olivier Schaerer, Kristina Krempaska, Silvia T Erni, R. Maarten van Dijk, Christian Moser, Mario Amacker, Rebecca Agaatha Maria Blom |
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| Přispěvatelé: | University of Zurich, Blank, Fabian |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
RNA viruses Influenza Viruses 10017 Institute of Anatomy Physiology medicine.medical_treatment Cellular differentiation lcsh:Medicine Pathology and Laboratory Medicine Epithelium White Blood Cells Spectrum Analysis Techniques Animal Cells Immune Physiology Medicine and Health Sciences lcsh:Science Immune Response Innate Immune System Multidisciplinary Cell Differentiation Flow Cytometry 3. Good health Virosome Cell biology Cytokine Spectrophotometry Medical Microbiology Viral Pathogens Viruses Cytokines Cytophotometry Cellular Structures and Organelles Cellular Types Anatomy Pathogens Research Article Immune Cells Immunology 610 Medicine & health 1100 General Agricultural and Biological Sciences Biology Research and Analysis Methods Microbiology 03 medical and health sciences Immune system Antigen 1300 General Biochemistry Genetics and Molecular Biology medicine Vesicles Progenitor cell Microbial Pathogens 1000 Multidisciplinary Blood Cells Macrophages lcsh:R Organisms Biology and Life Sciences Epithelial Cells Cell Biology Molecular Development 030104 developmental biology Biological Tissue Cell culture Immune System Liposomes 570 Life sciences biology lcsh:Q Nanocarriers Developmental Biology Orthomyxoviruses |
| Zdroj: | PLoS One Blom, Rebecca Agaatha Maria; Erni, Silvia T; Krempaská, Kristína; Schaerer, Olivier; van Dijk, R Maarten; Amacker, Mario; Moser, Christian; Hall, Sean; Von Garnier, Christophe; Blank, Fabian (2016). A Triple Co-Culture Model of the Human Respiratory Tract to Study Immune-Modulatory Effects of Liposomes and Virosomes. PLoS ONE, 11(9), e0163539. Public Library of Science 10.1371/journal.pone.0163539 PLoS ONE PLoS ONE, Vol 11, Iss 9, p e0163539 (2016) PLoS ONE, 11 (9) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0163539 |
| Popis: | The respiratory tract with its ease of access, vast surface area and dense network of antigen-presenting cells (APCs) represents an ideal target for immune-modulation. Bio-mimetic nanocarriers such as virosomes may provide immunomodulatory properties to treat diseases such as allergic asthma. In our study we employed a triple co-culture model of epithelial cells, macrophages and dendritic cells to simulate the human airway barrier. The epithelial cell line 16HBE was grown on inserts and supplemented with human blood monocyte-derived macrophages (MDMs) and dendritic cells (MDDCs) for exposure to influenza virosomes and liposomes. Additionally, primary human nasal epithelial cells (PHNEC) and EpCAM+ epithelial progenitor cell mono-cultures were utilized to simulate epithelium from large and smaller airways, respectively. To assess particle uptake and phenotype change, cell cultures were analyzed by flow cytometry and pro-inflammatory cytokine concentrations were measured by ELISA. All cell types internalized virosomes more efficiently than liposomes in both mono- and co-cultures. APCs like MDMs and MDDCs showed the highest uptake capacity. Virosome and liposome treatment caused a moderate degree of activation in MDDCs from mono-cultures and induced an increased cytokine production in co-cultures. In epithelial cells, virosome uptake was increased compared to liposomes in both mono- and co-cultures with EpCAM+ epithelial progenitor cells showing highest uptake capacity. In conclusion, all cell types successfully internalized both nanocarriers with virosomes being taken up by a higher proportion of cells and at a higher rate inducing limited activation of MDDCs. Thus virosomes may represent ideal carrier antigen systems to modulate mucosal immune responses in the respiratory tract without causing excessive inflammatory changes. ISSN:1932-6203 |
| Databáze: | OpenAIRE |
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