Initiation of transcription is temperature-dependent in an E. coli mutant with ts adenylate kinase
| Autor: | Joan L. Suit, Salvador E. Luria, Charles A. Plate |
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| Rok vydání: | 1975 |
| Předmět: |
Transcription
Genetic Mutant Biophysics Ornithine transcarbamylase Adenylate kinase Biochemistry Adenosine Triphosphate Species Specificity Transcription (biology) Escherichia coli Protein biosynthesis AK activity Molecular Biology Ornithine Carbamoyltransferase biology Adenylate Kinase Phosphotransferases Temperature Cell Biology biology.organism_classification Molecular biology Galactosidases Kinetics Enzyme Induction Mutation Related strain Bacteria |
| Zdroj: | Biochemical and Biophysical Research Communications. 67:353-358 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(75)90323-x |
| Popis: | In a mutant of E. coli with temperature-sensitive adenylate kinase (AK) activity the synthesis of β-galactosidase or ornithine transcarbamylase cannot be induced at the restrictive temperature of 40°, but is induced normally at 27°. However, bulk protein synthesis, including the synthesis of pre-induced β-galactosidase, continues for several minutes after a temperature shift from 27° to 40°. These effects of temperature shift mimic the effects of rifampin on a related strain with normal AK activity. The results are interpreted as indicating that the mutant bacteria at the restrictive temperature are blocked in initiation of transcription. |
| Databáze: | OpenAIRE |
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