Immunization of male but not female mice with the sperm-specific isozyme of lactate dehydrogenase (LDH-C4) impairs fertilization in vivo
| Autor: | James W. Overstreet, Catherine A. VandeVoort, Patricia A. O'Hern, Erwin Goldberg, Ryan P. Mcguinness, Cherrie A. Mahi-Brown |
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| Rok vydání: | 1990 |
| Předmět: |
Male
medicine.medical_specialty animal diseases Immunology chemical and pharmacologic phenomena Semen Orchitis Testicle Biology chemistry.chemical_compound Mice Human fertilization Lactate dehydrogenase Internal medicine Testis medicine Immunology and Allergy Animals Contraception Immunologic Immunocontraception L-Lactate Dehydrogenase Obstetrics and Gynecology biochemical phenomena metabolism and nutrition Sperm Spermatozoa Sperm Transport Isoenzymes medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Immunization Fertilization bacteria Oviduct Female |
| Zdroj: | American journal of reproductive immunology (New York, N.Y. : 1989). 24(1) |
| ISSN: | 1046-7408 |
| Popis: | The goals of this study were to determine the site at which fertility is impaired in mice immunized with LDH-C4 and to determine whether immunization of both males and females would have a greater antifertility effect than immunization of one sex alone. Mice were immunized with LDH-C4 in two systemic doses in Freund's adjuvants and two gastric doses in bicarbonate buffer. The presence of anti-LDH-C4 antibodies in uterine fluid was confirmed. Male and female mice were assigned to four blocks in which either the males, the females, both, or neither were immunized. Oviducts were viewed directly 1 h after mating for the presence of sperm. No significant effect of immunization on sperm transport to the oviduct could be demonstrated. Fertilization was evaluated 4 h after mating. It was found that immunization of males, but not females, impaired fertilization (19.6% versus 50.8% of the oocytes penetrated in 17 and 19 females, respectively). Orchitis was found histologically in 43.8% of the immunized males and 10% of the control males. |
| Databáze: | OpenAIRE |
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