Bone marrow concentrate-induced mesenchymal stem cell conditioned medium facilitates wound healing and prevents hypertrophic scar formation in a rabbit ear model

Autor: Chih Hung Chou, Oscar K. Lee, Chiung Wen Hu, Yi Wen Tseng, Hsien Da Huang, Ko Hsun Liao, Chih Yung Chiou, Jui Yung Yang, Shiow Shuh Chuang, Kuan Chun Lan, Ching Hsuan Hu, Chun San Tai
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Cicatrix
Hypertrophic
Medicine (miscellaneous)
Biochemistry
Genetics and Molecular Biology (miscellaneous)
Hypertrophic scar
Transforming Growth Factor beta1
Extracellular matrix
lcsh:Biochemistry
Mice
03 medical and health sciences
0302 clinical medicine
Bone Marrow
Fibrosis
medicine
Animals
lcsh:QD415-436
Myofibroblasts
Fibroblast
Cell Proliferation
Skin
Mesenchymal stem cell-conditioned medium
Mice
Inbred BALB C
Wound Healing
lcsh:R5-920
integumentary system
Chemistry
Bone marrow concentrate
Research
Mesenchymal stem cell
Ear
Mesenchymal Stem Cells
Cell Biology
Fibroblasts
medicine.disease
Extracellular Matrix
Cell biology
030104 developmental biology
medicine.anatomical_structure
Culture Media
Conditioned
030220 oncology & carcinogenesis
Molecular Medicine
Female
Rabbits
Stem cell
Wound healing
lcsh:Medicine (General)
Myofibroblast
Zdroj: Stem Cell Research & Therapy, Vol 10, Iss 1, Pp 1-13 (2019)
Stem Cell Research & Therapy
ISSN: 1757-6512
DOI: 10.1186/s13287-019-1383-x
Popis: Background Hypertrophic scars (HSs) are formed via an aberrant response to the wound healing process. HSs can be cosmetic or can result in functional problems. Prolonged proliferation and remodeling phases disrupt wound healing, leading to excessive collagen production and HS formation. However, there are currently no satisfactory drugs to prevent HS formation. Mesenchymal stem cell (MSC) conditioned medium (CM) has therapeutic effects on wound healing and preventing HS formation. Bone marrow concentrate (BMC) contains various growth factors and cytokines that are crucial for regeneration and has been applied in the clinical setting. In this study, we evaluated the effects of BMC-induced MSC CM on HS formation in a rabbit ear model. Methods We established a rabbit ear wound model by generating full-thickness wounds in the ears of rabbits (n = 12) and treated wounds with MSC CM, BMC CM, or BMC-induced MSC CM. Dermal fibroblasts from human hypertrophic scar were stimulated with transforming growth factor beta 1 (TGF-β1) for 24 h and cultured in each culture medium for 72 h. We measured the hypertrophic scar (HS) formation during the skin regeneration by measuring the expression of several remodeling molecules and the effect of these conditioned media on active human HS fibroblasts. Results Our results showed that BMC-induced MSC CM had greater antifibrotic effects than MSC CM and BMC CM significantly attenuated HS formation in rabbits. BMC-induced MSC CM accelerated wound re-epithelization by increasing cell proliferation. Additionally, BMC-induced MSC CM also inhibited fibrosis by decreasing profibrotic gene and protein expression, promoting extracellular matrix turnover, inhibiting fibroblast contraction, and reversing myofibroblast activation. Conclusions BMC-induced MSC CM modulated the proliferation and remodeling phases of wound healing, representing a potential wound healing agent and approach for preventing HS formation. Electronic supplementary material The online version of this article (10.1186/s13287-019-1383-x) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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