Identification of a cluster of HIV-1 controllers infected with low replicating viruses
| Autor: | Lidia Ruiz, Isabel Olivares, Lorena Martínez-Prats, Carmen Rodríguez, Cecilio López-Galíndez, Maria Pernas, Tamara Alvaro-Cifuentes, Rafael Delgado, Virginia Sandonis, Jorge del Romero, Eulalia Grau, Rosa Fuentes, Concepción Casado |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Fundación para la Investigación y la Prevención del Sida en España, Instituto de Salud Carlos III |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Male
Sequence analysis Science HIV Infections Biology Virus Replication Genes env Virus HIV Long-Term Survivors Viral envelope Genotype Humans Allele Gene Alleles Phylogeny Genetics Multidisciplinary env Gene Products Human Immunodeficiency Virus Virology Viral replication Spain Viral evolution Disease Progression HIV-1 Medicine Female Research Article |
| Zdroj: | PLoS ONE, Vol 8, Iss 10, p e77663 (2013) PLoS ONE Repisalud Instituto de Salud Carlos III (ISCIII) |
| ISSN: | 1932-6203 |
| Popis: | Long term non-progressor patients (LTNPs) are characterized by the natural control of HIV-1 infection. This control is related to host genetic, immunological and virological factors. In this work, phylogenetic analysis of the proviral nucleotide sequences in env gene from a Spanish HIV-1 LTNPs cohort identified a cluster of 6 HIV-1 controllers infected with closely-related viruses. The patients of the cluster showed common clinical and epidemiological features: drug user practices, infection in the same city (Madrid, Spain) and at the same time (late 70's-early 80's). All cluster patients displayed distinct host alleles associated with HIV control. Analysis of the virus envelope nucleotide sequences showed ancestral characteristic, lack of evolution and presence of rare amino-acids. Biological characterization of recombinant viruses with the envelope proteins from the cluster viruses showed very low replicative capacity in TZMbl and U87-CD4/CCR5 cells. The lack of clinical progression in the viral cluster patients with distinct combinations of protective host genotypes, but infected by low replicating viruses, indicate the important role of the virus in the non-progressor phenotype in these patients. Work in CNM is supported by grants [SAF 2007-61036 and 2010-17226] from MICINN Spain, by grants [36558/06, 36641/07, 36779/08, 360766/09] from Fundación para la investigación y prevención del SIDA en España (FIPSE) Spain, and in part by the RETIC de Investigación en SIDA [RD06/006/0036] of the Fondo de Investigaciones Sanitarias (FISss). Work in H12 de Octubre was financed by FIPSE [36749], and ISCIII-FIS [PI080806 and PI1101580] to RD. The HIV BioBank, integrated in the Spanish AIDS Research Network, is supported by the Instituto de Salud Carlos III, Spanish Health Department (Grant n° RD06/006/0035) and FIPSE. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. Sí |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|