Genetic characteristics of Theileria equi in zebras, wild and domestic donkeys in Israel and the Palestinian Authority
| Autor: | Ori Arieli, Yuval Gottlieb, Igal H. Horowitz, Amir Steinman, Monica L. Mazuz, Roni King, Sharon Tirosh-Levy |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Veterinary medicine Babesia caballi Asinus 030231 tropical medicine ved/biology.organism_classification_rank.species Animals Wild Parasitemia Real-Time Polymerase Chain Reaction Microbiology 18S ribosomal RNA 03 medical and health sciences Middle East 0302 clinical medicine Species Specificity Seroepidemiologic Studies Theileria parasitic diseases Genotype Genetic variation medicine Prevalence Animals Israel biology ved/biology Equidae medicine.disease biology.organism_classification Equus Theileriasis 030104 developmental biology Infectious Diseases Insect Science Parasitology Animals Zoo Female Donkey |
| Zdroj: | Ticks and tick-borne diseases. 11(1) |
| ISSN: | 1877-9603 |
| Popis: | Equine piroplasmosis (EP) is an important tick-borne disease of equids, caused by Theileria equi and Babesia caballi. It is endemic in most parts of the world, including Israel, and has clinical and economic consequences. This study was set to evaluate the presence of EP parasites in domestic donkeys and in wild equids in Israel and the Palestinian Authority (PA). To assess subclinical EP infection in 98 domestic donkeys (Equus africanus asinus), 9 Asiatic wild donkeys (Equus hemionus), 8 zebras (Equus quagga), 7 African wild donkeys (Equus africanus) and 5 mules, were tested using PCR and qPCR. Positive samples were characterized by amplification and sequencing of a 1600 bp fragment of the 18S rRNA gene. Babesia caballi was not detected in any of the animals. Theileria equi was detected in 32% of the donkeys, 89% of Asiatic wild donkeys, 57% of African wild donkeys, 62% of zebras and none of the mules. Parasitemia was low in all of the positive samples. Risk factors associated with infection in donkeys included one farm (Kiryat Gat) and animal sex (male). The sequences of the 18S rRNA gene from domestic donkeys were all similar, and belonged to the T. equi genotype D, similar to the genotype sequenced from horses in the same area, while sequences from wild donkeys were unique and belonged to the T. equi genotype A. Verification of the T. equi genotype in zebras could not be concluded, suggesting major genetic variation. |
| Databáze: | OpenAIRE |
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